The CLSI Antifungal Subcommittee followed the M23-A2 "blueprint" to develop interpretive MIC breakpoints for anidulafungin, caspofungin, and micafungin against Candida species. MICs of < or = 2 microg/ml for all three echinocandins encompass 98.8 to 100% of all clinical isolates of Candida spp. without bisecting any species group and represent a concentration that is easily maintained throughou...

Antibiotic resistance in gram negative bacteria is increasing worldwide in both outpatients as well as hospitalized patients. Most bacterial isolates carry resistance determinants for extended spectrum beta lactamases (ESBL) production on plasmids that can easily spread from organism to organism. This study was conducted to compare the rate of detection of ESBL positive organisms by different m...

This study aimed to assess the interlaboratory reproducibility at four university hospital laboratories in the southeast region of France of the Etest technique for the determination of caspofungin (CAS) and amphotericin B (AMB) MICs and to compare it to the CLSI broth microdilution reference method. Consecutive clinical yeast isolates (n = 198) were included in the study. AMB and CAS MICs were...

BACKGROUND/PURPOSE To document the trends of sensitivity and to find whether it is necessary to change antibiotics in selected patients according to the sensitivity test results in our clinical practice. METHODS We collected urine culture results from 0-18-year-old patients in the National Taiwan University Hospital from January 1, 2003 to October 31, 2012. Their medical chart was reviewed to...

: Combined clinical pretest probability (PTP) and D-dimer testing have great diagnostic value for pulmonary embolism exclusion. To harmonize performance levels of D-dimer assays available on the market, the Clinical and Laboratory Standard Institute (CLSI) has published a guideline, endorsed by the US Food and Drug Administration (FDA). Such guideline specifies the ideal D-dimer assay character...

We applied the new clinical breakpoints (CBPs) of the Clinical and Laboratory Standards Institute (CLSI) to a multicenter study to determine the antifungal susceptibility of bloodstream infection (BSI) isolates of Candida species in Korea, and determined the relationship between the frequency of antifungal-resistant Candida BSI isolates and antifungal use at hospitals. Four hundred and fifty BS...

INTRODUCTION The phlebotomists' procedures are a still source of laboratory variability. The aim of this study was to verify the efficacy of minor modification in procedure for collection of diagnostic blood specimens by venipuncture from CLSI H03-A6 document is able to reduce the tourniquet application time. MATERIALS AND METHODS Thirty phlebotomists were invited to participate. Each phlebot...

Reference interval limits for cancer biomarkers in geriatrics are rare because priority is given to the development of reference those age range 18–60 years, which normally used clinical trials study. The aim this study was therefore develop gender and age-specific markers CA19-9, CEA, CA 15-3, 125, PSA adults Taita-Taveta County, Kenya, using CLSI CA28-A3 guideline. This prospective cross-sect...

Abstract Background Recent studies have indicated the presence of IC arising within zone inhibition during fosfomycin DD testing. CLSI and EUCAST contradicting recommendations for interpreting these IC; recommends considering when results while ignoring them. Although testing is approved only use with EC per both organizations, it often used non-EC organisms despite neither organization publish...

Abstract Background The existence of multidrug-resistant organisms, including extended-spectrum beta-lactamases (ESBLs), is on rise across the globe and becoming a severe problem. Knowledge prevalence antibiogram profile such isolates essential to develop an appropriate treatment methodology. This study aimed Gram-negative exhibiting ESBL at tertiary care hospital their profile. Methods A cross...