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Erythrocyte glucose-6-phosphate dehydrogenase activity is measured with a centrifugal analyzer by use of a commercial reagent kit and of the reaction glucose-6-phosphate + NADP+ leads to 6-phosphogluconolactone + NADPH. Rate of production of NADPH is measured and related to hemoglobin concentration. Maleimide is added to inhibit further production of NADPH in a secondary reaction by endogenous ...
Pectinolytic enzymes are an important tool for sustainable food production, with a wide range of applications in processing technologies as well the extraction bioactive compounds from pectin-rich raw materials. In present study, we immobilized commercial pectinase preparation onto pellet and thread shaped nylon 6/6 carriers assessed its stability reusability. Five preparations were tested diff...
A systematic study of the carbohydrate-metabolizing enzyme systems of the Morris hepatoma 51~3 was carried out, and results were interpreted in the light of normal liver data and in comparison with previously established values of Novikoff hepatoma. The cellularity of the Morris tumor was in the same range, whereas the nitrogen content of the average cell was significantly decreased, as compare...
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