background: toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. the classical diagnosis of toxoplasmosis relies on serological methods. detection of parasite or its components could be useful tool for early diag­nosis of the infection. methods: fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of t. gondii rh strain. five of them sacrifi...

OBJECTIVE There have been a large number of reports on alterations in the serum level of keratan sulphate (KS), a potential marker of articular cartilage degeneration in patients with arthropathy. Such studies have commonly employed ELISA using the anti-KS monoclonal antibody 1/20/5D4 (5D4-ELISA) to determine KS levels. Recently, a highly sensitive KS ELISA (HS-ELISA) kit has been developed, al...

background and aims: measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including iran. methods: the present study was performed to evaluate the immune status agai...

background: the diagnosis of toxocariasis heavily depends on immunological tests because the number of parasites is usually few in infected tissues, unless they migrate into an organ such as eye. in general, patients with ocular toxocariasis have serum anti- t canis antibody titres that are significantly lower than those with visceral toxocariasis. objective: to diagnose the asymptomatic toxoca...

herein, we presented the first report on bluetongue (bt) disease in 10 pregnant camels in a herd fromkerman province, iran. all sera samples were tested serologically (agid, c-elisa). we also used the razi-bk cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ece) to culture and isolate the bt virus. efforts to culture and isolation of...

to develop an elisa technique for the detection of antibodies against babesia ovis, the infectederythrocytes were lysed and the supernatant soluble antigen, after sonication and ultracentrifugation of thelysate was used as antigen. optimal dilution of the antigen was determined by checkerboard titrations, usingpositive and negative control sera. a correlation of 85% was observed between the res...