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A gram-positive coccus, presently named Micrococcus mucilaginosus incertae sedis, was isolated from 14 blood cultures from a patient with endocarditis. The first positive blood culture was drawn 5 days after the patient underwent cardiac catheterization.
The respiratory tract contains numerous antimicrobial factors necessary for normal innate pulmonary defense. Although many of these molecules reside in airway surface liquid (ASL) simultaneously, little information exists concerning antagonistic, additive, or synergistic interactions. Since both cationic lysozyme and anionic antimicrobial peptides (AP) are found in high concentrations in ASL, t...
Here, we present the draft genome sequence of the actinobacterium Curtobacterium sp. strain UCD-KPL2560, which was isolated from the running surface of an indoor track field house in Medford, MA, USA (42.409716°N, -71.115169°W). The genome assembly contains 3,480,487 bp in 156 contigs.
When a culture of the temperature-sensitive DNA mutant Micrococcus radiodurans tsI is irradiated with a sublethal dose of ultraviolet or ionizing radiation and is plated immediately, all the bacteria give rise, after 36 h incubation, to colonies identical to those derived from unirradiated bacteria. However, when the irradiated population is held at its restrictive temperature (39 degrees C) (r...
Pruess, David L. (University of Wisconsin, Madison), and Marvin J. Johnson. Enzymatic deacylation of S(35)-benzylpenicillin. J. Bacteriol. 90:380-383. 1965.-S(35)-benzylpenicillin, penicilloic acid, and penilloic acid were deacylated by cell suspensions of Escherichia coli and Micrococcus roseus. Both cultures deacylated penicillin most rapidly and penilloic acid least rapidly. The deacylase ac...
Elder, Robert L. (Johns Hopkins University, Baltimore, Md.), and Roland F. Beers, Jr. Nonphotoreactivating repair of ultraviolet light-damaged Micrococcus lysodeikticus cells. J. Bacteriol. 89:1225-1230. 1965.-Micrococcus lysodeikticus cells are highly resistant to ultraviolet radiation. The shape of the dose-survival curve is consistent with the presence of a repair mechanism. The presence of ...
Metallic copper alloys have recently attracted attention as a new antimicrobial weapon for areas where surface hygiene is paramount. Currently it is not understood on a molecular level how metallic copper kills microbes, but previous studies have demonstrated that a wide variety of bacteria, including Escherichia coli, Staphylococcus aureus, and Clostridium difficile, are inactivated within min...
Studies on the process of nitrite reduction by cell-free extracts of denitrifying bacteria could materially contribute to our knowledge of its mechanism. Such investigations have so far been limited to those of Yamagata (1) with Bacillus pyocyaneus (Pseudomonas aeruginosa) and of Robinson et al. (‘2) with Micrococcus haZodenitri$cans. Neither of these authors has examined the products of the re...
Nonhistone proteins fractionated from normal rat liver and Walker carcinosarcoma can stimulate chromatin-templated RNA synthesis in vitro. This has been demonstrated by using RNA polymerase prepared from rat liver. Walker tumor, or Micrococcus lutcus. The RNA isolated from these fractionated nonhistone proteins does not activate transcription from chromatin. As judged by DNA-RNA hybridization s...
Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) is rapidly becoming a routine method for identification of microorganisms in the United States. Early studies showed strong performance for both commonly used systems, with identification rates of 90% and misidentification rates of 2% in some studies (1– 4). One of the major advantages of using MALDI-T...
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