Our objective was to determine the efficacy of adenoviral-mediated gene therapy with wild-type p53 or p21 in human breast cancer cells and investigate interactions with radiation and chemotherapy. Two human breast cancer cell lines, MDA-MB-231 and MDA-MB-435, both with p53 mutations, were transduced with adenoviral vectors containing wild-type p53 (Ad5CMV-p53) or p21/WAF1/Cip1 (Ad5CMV-p21), and...

هدف: سرطان از جمله مهمترین بیماری­های قرن حاضر است که افراد بسیاری در سراسر جهان به آن مبتلا می­باشند و ضمن افزایش شیوع این بیماری، هنوز درمان مناسبی برای پیدا نشده است. بین پستان شایع­ترین همچنین علت اصلی مرگ میر ناشی زنان محققان بدنبال یافتن راهکارهای درمانی سرطان، ترکیبات دارویی را علیه سرطان­های مختلف سنتز کرده اثرات آ­نها مورد مطالعه قرار می­دهند. سورافنیب یک مهار کننده­ی اوره مولتی­کیناز ...

A total of forty novel glycyrrhetinic acid (GA) derivatives were designed and synthesized. The cytotoxic activity of the novel compounds was tested against two human breast cancer cell lines (MCF-7, MDA-MB-231) in vitro by the MTT method. The evaluation results revealed that, in comparison with GA, compound 42 shows the most promising anticancer activity (IC50 1.88 ± 0.20 and 1.37 ± 0.18 µM for...

Supplementary Figure 1. Total number of MDA-MB-231 cells is conserved in four micropattern design. (a): Green fluorescence protein expression measured by plate reader after cell lysis served as an accurate measure of cell number in the culture. (b): MDA-MB-231 cells harvested from four micropatterns had similar amount of cells, with none of the groups having statistical difference from the othe...

Актуальність. Куркумін і кверцетин виявилися дуже ефективними проти раку молочної залози. Однак повністю їх протипухлинні механізми невідомі. У цьому дослідженні вивчено вплив куркуміну та кверцетину на ріст лінії клітин залози людини MCF-7 MDA-MB-231 шляхом регуляції miR-632 miR-137. Матеріали методи. Клітини обробляли куркуміном кверцетином у різних розведеннях протягом 24 48 годин. Життєздат...

UNLABELLED The Auger electron-emitting radiopharmaceutical 111In-diethylenetriaminepentaacetic acid human epidermal growth factor (111In-DTPA-hEGF) binds the epidermal growth factor receptor (EGFR), is internalized, and translocates to the nucleus. The purpose of this study was to investigate the relationship between EGFR expression, DNA damage, and cytotoxicity in cells exposed to 111In-DTPA-h...

Methods. Cells and reagents We purchased MDA-MB-231 cells from ATCC and primary IMR90, HDF and primary HUVEC cells from Lonza. MDA-MB-231, IMR-90, HDF and LLC cells were cultured in Eagle’s minimal Essential medium (MEM) from Lonza supplemented with 10% fetal bovine serum (FBS) and 1% GlutaMax (both from Invitrogen). Primary HUVEC cells were cultured in endothelial growth medium (EGM-2, Lonza)....

BACKGROUND Twist1 is involved in tumor initiation and progression, which especially contributes to tumor invasion and metastasis. Wogonoside is the main in-vivo metabolite of wogonin, and it is also a natural product with potential treatment effects against cancer. METHODS In this study, we investigated the in-vitro anti-invasion and in-vivo anti-metastasis effects of wogonoside on breast can...

Abelson interactor protein-1 (ABI-1) is an adaptor protein involved in actin reorganization and lamellipodia formation. It forms a macromolecular complex containing Hspc300/WASP family verprolin-homologous proteins 2/ABI-1/nucleosome assembly protein 1/PIR121 or Abl/ABI-1/WASP family verprolin-homologous proteins 2 in response to Rho family-dependent stimuli. Due to its role in cell mobility, w...

We showed previously that breast cancer chemoprevention with benzyl isothiocyanate (BITC) in MMTV-neu mice was associated with induction of E-cadherin protein in vivo. Loss of E-cadherin expression and induction of mesenchymal markers (e.g. vimentin) are biochemical hallmarks of epithelial-mesenchymal transition (EMT), a developmental process implicated in progression of cancer to aggressive st...