Proteins bind and control mRNAs, directing their localization, translation and stability. Members of the PUF family of RNA-binding proteins control multiple mRNAs in a single cell, and play key roles in development, stem cell maintenance and memory formation. Here we identified the mRNA targets of a S. cerevisiae PUF protein, Puf5p, by ultraviolet-crosslinking-affinity purification and high-thr...

Post-transcriptional regulation of gene expression plays a crucial role in many processes. In cells, it is mediated by diverse RNA-binding proteins. These proteins can influence mRNA stability, translation, and localization. The PUF protein family (Pumilio and FBF) is composed of RNA-binding proteins highly conserved among most eukaryotic organisms. Previous investigations indicated that they c...

PUF proteins regulate translation and mRNA stability throughout eukaryotes. Using a cell-free translation assay, we examined the mechanisms of translational repression of PUF proteins in the budding yeast Saccharomyces cerevisiae. We demonstrate that the poly(A)-binding protein Pab1p is required for PUF-mediated translational repression for two distantly related PUF proteins: S. cerevisiae Puf5...

Genome-wide identification of mRNAs regulated by RNA-binding proteins is crucial to uncover post-transcriptional gene regulatory systems. The conserved PUF family RNA-binding proteins repress gene expression post-transcriptionally by binding to sequence elements in 3'-UTRs of mRNAs. Despite their well-studied implications for development and neurogenesis in metazoa, the mammalian PUF family mem...

Physical Unclonable Functions (PUFs) are physical structures that are hard to clone and have a unique challenge-response behaviour. The term PUF was coined by Pappu et al. in 2001. That work triggered a lot of interest, and since then a substantial number of papers has been written about the use of a wide variety of physical structures for different security purposes such as identification, aut...

A Physical Unclonable Function (PUF) is a device with unique behaviour that hard to clone hence providing secure fingerprint. variety of PUF structures and PUF-based applications have been explored theoretically as well being implemented in practical settings. Recently, the inherent unclonability quantum states has exploited derive analogue new proposals for implementation PUF. We present first...

The internet of things (IoT) describes the network physical objects equipped with sensors and other technologies to exchange data devices over Internet. Due its inherent flexibility, field-programmable gate array (FPGA) has become a viable platform for IoT development. However, various security threats such as FPGA bitstream cloning intellectual property (IP) piracy have major concern this devi...

Physical Unclonable Function(PUF) is a hardware security primitive with desirable feature of low-cost.When combination MAX and MIN bitwise operations are used in PUF recompositionits resilience against model attacks was expected to be improved markedly.To our knowledge, there no explicit evaluation this recomposition; thus, study the first evaluate uniformity reliability (x,y,z)-OAX-PUF.Compare...

Physical Unclonable Function (PUF) is a promising lightweight hardware security primitive that can extract device fingerprints for encryption or authentication. However, extracting from either the chip board individually has flaws and cannot provide system-level security. This paper proposes new Chip-PCB hybrid PUF(CPR PUF) in which Weak PUF on PCB combined with Strong inside to generate massiv...