A complement fixation test, using round-bottomed microtitration plates and an 8 channel microdiluter, based on that used for brucellosis by Herr, Huchzermeyer, Te Brugge, Williamson, Roos & Schiele, 1985, has been developed for use on the sera of horses to detect antibodies to the contagious equine metritis organism. The results with 2 known positive sera tested 116 times in 27 separate tests w...

A Laboratory Branch Task Force of the National Communicable Disease Center has proposed a standardized complement fixation procedure (LBCF) and an adaptation of this to microtitration techniques (MT) as uniform methods for performing complement fixation (CF) tests. A common procedure should make CF results from one laboratory more comparable to another. In addition, it would be preferable if th...

Antigens capable of fixing complement specifically with the appropriate antibodies have been prepared from brain tissue of hamsters and mice infected with the viruses of St. Louis, Japanese, Western, and Eastern encephalitis, and with the West Nile virus. The antigens were freed of the material which reacts with normal serum by means of centrifugation at relatively high speed. In addition, the ...

Complement-fixing antibody was detected in 13 of 16 patients with histological and/or culture evidence of infection with Nocardia species. The antigen used was a filtrate of soluble substances secreted into liquid growth medium by cultures of N. asteroides. Apparent false positives reactions were found in three of three patients with leprosy and two of five patients with tuberculosis--results ...

The prolonged extraction of the tubercle bacillus with boiling ethyl alcohol, followed by one or more reprecipitations by chilling the hot alcoholic solution, easily yields a preparation very active as antigen in the complement fixation reaction. This preparation gives a precipitation reaction with high dilution of the normal blood serum of a number of species. The precipitation reaction presen...

In a previous publication (Sen Gupta, 1943), the results of the complement-fixation test using the original Witebsky, Klingenstein, Kuhn (WKK) antigen were reported. Reference was also made about the preparation of the antigen according to the WKK method. It was shown that a complement-fixation test of marked degree of ' specificity' for kala-azar had been evolved. In order to investigate furth...

The relationship between antibody titres in the microtitration serum agglutination test and the complement fixation test in bovine brucellosis is described. For low and high MSAT values there is good agreement between the 2 tests. This is not the case for MSAT values between 54 and 338 IU/ml. For practical reasons, results falling into this category cannot all be repeated. Repetitions are so st...

SHIRACHI , R., SLTKENO, N., UMMENAT, T. and ISRIDA, N. Heavy-density Particles of Australia Antigen Detected in a Human Plasma Pool. Tohoku J. exp. Med., 1972, 107 (4), 359-365„ŸBiophysical study of Australia antigen (Au) was made with 5 specimens including 3 plasmas and 2 plasma pools which had been known to be Au carriers. A fraction which could be detected both by comple ment fixation test a...