The micromechanical properties of spider air flow hair sensilla (trichobothria) were characterized with nanometre resolution using surface force spectroscopy (SFS) under conditions of different constant deflection angular velocities theta (rad s(-1)) for hairs 900-950 microm long prior to shortening for measurement purposes. In the range of angular velocities examined (4 x 10(-4) - 2.6 x 10(-1)...

BACKGROUND In response to genotoxic stress, cells activate checkpoint pathways that lead to a transient cell cycle arrest that allows for DNA repair or to apoptosis, which triggers the demise of genetically damaged cells. RESULTS During positional cloning of the C. elegans rad-5 DNA damage checkpoint gene, we found, surprisingly, that rad-5(mn159) is allelic with clk-2(qm37), a mutant previou...

[Objective] To study the inhibitory effect of 3′ untranslated region (UTR)-targeted artificial microRNA (amiRNA) against porcine reproductive and respiratory syndrome virus (PRRSV) replication in porcine alveolar macrophages (PAM). [Methods] Recombinant adenovirus (rAd) expressing the 3′ UTR-targeted or control amiRNA and green fluorescent protein (GFP) reporter gene were generated by transfect...

The yield of chromosome aberrations was studied in Go human peripheral blood lymphocytes following in vitro exposure to 100, 250 and 500 rad of caesium-137 gamma radiation. The doses were given at various rates up to 400 rad per h with the longest exposures lasting for approx. 50 h. At 500 and 250 rad the dicentric yields fell by 66 and 64% when the dose rate was reduced from 400 to 10 rad per ...