SUMMARY The complete nucleotide sequence of the genomic RNA of the potyvirus potato virus Y strain N (PVYn) was obtained from cloned cDNAs. This sequence is 9704 nucleotides long and can encode a polyprotein of 3063 amino acids. The positions of the cleavage sites at the N terminus of the capsid and cytoplasmic inclusion proteins have been determined. Other putative protein cleavage sites have ...

Tomato spotted wilt virus (TSWV) is a Tospovirus affecting field tomato crops worldwide. The virus is transmitted by some species of thrips. The most damaging vectors in Italy are Frankliniella occidentalis and Thrips tabaci. Only the first instar larvae can acquire the virus from an infected plant. After acquisition, the virus replicates in the vector, and the viruliferous thrips is capable of...

Aim. Screening of vegetable crops for mixed viral infections caused by 8 viruses, and evaluation relative share different combinations pathogens using serological methods plant virus diagnostics. Methods. Double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was performed detection antigens. Results. For this study, the samples from plants with virus-like symptoms were collecte...