The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual cells, effectively resulting in knock-out of targeted genes. It thus promises to be an ideal candidate for application in neuroscience where constitutive genetic modificatio...

of Detonation (Knock) Using a Wavelet to Create a Chart for a Petrol Engine’s Characteristics Agus Sujono , R. Soekrisno , Eka Firmansyah , Oyas Wahyunggoro #4 # Faculty of Engineering, Sebelas Maret University (UNS), PhD student of Electrical Engineering, Gadjah Mada University (UGM) Surakarta , Indonesia # Faculty of Engineering, UGM, Yogyakarta, Indonesia 1 [email protected] 4 oyas@ug...

Fluorescent proteins and epitope tags can reveal protein localization in cells animals, yet the large size of many hinders efficient genome targeting. Accordingly, studies have relied on characterizing overexpressed proteins, which might not recapitulate endogenous activities. Here, we present two strategies for higher throughput production reporters mice, focusing blastocyst model development....

This paper asks what sorts of ethical caution ought to attach increasingly data-driven approaches understanding the brain. is taken be an important question especially owing a likely near future neuromonitoring and neuromodulation devices with applications in psychiatry. The explores this by i) sketching concept ‘digital twin,’ ii) drawing schematic picture ‘brain datafication’ general, iii) de...

The CCAAT/enhancer binding protein A (C/EBPA) is vital for establishing normal hepatic energy homeostasis and moderating hepatocellular growth. CEBPA loss-of-function mutations identified in acute myeloid leukemia patients support a tumor suppressor role for C/EBPA. Recent work showed reductions of C/EBPA levels in human hepatocellular carcinoma with the reductions correlating to tumor size and...

BACKGROUND Manganese superoxide dismutase (MnSOD) is a mitochondrial antioxidant enzyme that is down-regulated in a majority of cancers. Due to this observation, as well as MnSOD's potent antioxidant enzymatic activity, MnSOD has been suggested as a tumor suppressor for over 30 years. However, testing this postulate has proven difficult due to the early post-natal lethality of the MnSOD constit...

The liver is the major organ responsible for the uptake of modified low-density lipoprotein (LDL) from the blood circulation, with endothelial and Kupffer cells as major cellular uptake sites. Scavenger-receptors, which include various classes, are held responsible for this uptake. Mice deficient in scavenger-receptor class A types I and II were created and the fate of acetylated LDL (Ac-LDL) i...

The resonances of SI engine combustion chambers are slightly excited during normal combustion but strongly excited by knock. In order to avoid knocking combustions extensive knowledge about knock and its effects is necessary. In this paper the combustion chamber of a serial production engine is modeled by finite elements. Modal analyses are performed in order to gain information about the reson...

In this paper we consider a basic problem in the layout of VLSI-circuits, the channel-routing problem in the knock-knee mode. We show that knock-knee channel routing with 3-terminal nets is NP-complete and thereby settling a problem that was open for more than a decade. In 1987, Sarrafzadeh showed that knock-knee channel routing with 5-terminal nets is NP-complete (Sarrafzadeh, 1987). Furthermo...

In order to detect knock in spark ignition engines, usually structure-borne sound signals measured by acceleration sensors mounted on the engine housing are analyzed. Earlier investigations have shown that using linear, time variant filtered structure-borne sound signals as approximated pressure signal instead improves knock detection significantly. But this method is computationally too expens...