Studies that aim to characterise the susceptibility of ecologically relevant and non-model fish polar cod (Boreogadus saida) model carcinogens are required. Polar were exposed under laboratory conditions for six months control, 0.03 μg BaP/g fish/week 0.3 dietary benzo(a)pyrene (BaP), a reference carcinogen. The concentrations 3-OH-BaP bile metabolite transcriptional responses genes involved in...

a fast and simple modified quechers extraction method was developed for determination of benzo[a]pyrene (bap) in 137 traditional (sangak), semi-industrial (sangak) and industrial bread samples using spiked calibration curves by gc/ms. sample preparation includes extraction of bap into acetone followed by cleanup with dispersive solid phase extraction. the limit of detection and limit of quantif...

Micropropagation of three genotypes of Indian mustard [Brassica juncea (L.) Czern.] using 7-days old seedling-derived transverse thin cell layer (tTCL) explants was accomplished. The genotype, explant source and addition of silver nitrate to the medium significantly influenced shoot bud induction. MS medium with 26.6 μM of 6-Benzylaminopurin (BAP) and 3.22 μM of 1-naphtaleneacetic acid (NAA) wa...

Lemongrass (Cymbopogon citratus) essential oil (EO) is a major source of bioactive compounds (BC) with anticancer activity such as ?-citral, limonene, geraniol, geranyl acetate, and ?-caryophyllene. Comparative studies about cytokinin effects on BC profiles in lemongrass are missing. Here, we evaluated four cytokinins (2iP, tZ, BAP, KIN) two different osmotic media, MS-N (3% sucrose, 3 g L?1 Ge...

Hypoxia promotes genetic instability and is therefore an important factor in carcinogenesis. We have previously shown that activation of the hypoxia responsive transcription factor HIFα can enhance the mutagenic phenotype induced by the environmental mutagen benzo[a]pyrene (BaP). To further elucidate the mechanism behind the ability of hypoxia to increase mutagenicity of carcinogens, we examine...

in the present study, an efficient protocol has been developed for callus induction and production of ra in callus culture of satureja khuzistanica for the first time. in vitro callus induction was achieved from young shoot tip explants cultured on ms and b5 media supplemented with different concentrations of iba (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins bap and kin...

Regulation of the balance between survival, proliferation, and apoptosis on carcinogenic polycyclic aromatic hydrocarbon (PAH) exposure is still poorly understood and more particularly the role of physiologic variables, including intracellular pH (pH(i)). Although the involvement of the ubiquitous pH(i) regulator Na(+)/H(+) exchanger isoform 1 (NHE1) in tumorigenesis is well documented, less is...

Lentiviral vectors are among the promising viral based-vectors in gene therapy applications, but the efficiency of their targeting needs to be improved. (Strept)avidin-biotin adaptor system is a novel approach to modify the lentiviral envelope for better targeting properties. Herein, we describe utilization of this adaptor system by designing a candidate envelope protein-bearing biotin acceptor...

OBJECTIVES Cytochrome P450 (CYP) 1A1 located in the membrane of endoplasmic reticulum is the most important enzyme in both activation and detoxification of carcinogenic benzo[a]pyrene (BaP), in combination with microsomal epoxide hydrolase (mEH). However, it is still not clearly explained how the electron transfer is mediated by NADPH:CYP oxidoreductase (POR), another component of the microsoma...

The adsorption of benzo(a)pyrene (BaP) on to three types of asbestos (chrysotile antophyllite, and amosite) and three types of manmade mineral fibres (MMMF) (rock wool, slag wool, and glass wool) in a physiological water solution was studied. Adsorption was determined from the decrease in the liquid concentration of BaP on the addition of the solid material. Results show that all the fibres wea...