نتایج جستجو برای: 1 cytochrome oxidase
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this study pursued the molecular identification of fish species from processed products for human consumption which, a priori, belonged to nine species. dna barcoding using direct sequencing of about 650 bp of the mitochondrial gene cytochrome oxidase subunit i (coi) revealed incorrect labeling in the three alaska pollack samples (11% of all samples). substitution of fish species constitutes se...
UNLABELLED Mycobacterium tuberculosis depends on aerobic respiration for growth and utilizes an aa3-type cytochrome c oxidase for terminal electron transfer. Cytochrome c maturation in bacteria requires covalent attachment of heme to apocytochrome c, which occurs outside the cytoplasmic membrane. We demonstrate that in M. tuberculosis the thioredoxin-like protein Rv3673c, which we named CcsX, i...
Deficiency of cytochrome c oxidase activity was established in a girl born to consanguineous parents. She showed symptoms of dysmaturity, generalized hypotonia, myoclonic seizures and progressive respiratory failure, leading to death on the seventh day of life. Structural abnormalities of the central nervous system consisted of severe cerebellar hypoplasia and optic nerve atrophy. Biochemical a...
Cytochrome oxidase probably occurs in the tissues of most plants and mediates a major portion of terminal respiration. Webster (11, 12) recently demonstrated the presence of a cytochrome oxidase in the tissues of 54 plant species, representing 23 families of dicotyledonous plants. Investigations in the plant physiology laboratory at Rutgers University have indicated the presence of a cytochrome...
A large-type sexannulate leech species, Orobdella ibukifukuyamai sp. nov. , from Kii-Oshima Island, Japan, is described based on morphologic and molecular data. Phylogenetic analyses using nuclear 18S rRNA, 28S histone H3, mitochondrial cytochrome c oxidase subunit I, tRNA Cys Met 12S Val 16S Leu NADH dehydrogenase 1 markers showed that O. formed a clade with the okanoi Nakano, 2016 yamaneae oc...
Cytochrome P-450 and cytochrome b(5) at levels of approximately 0.10 and 0.60 nanomole per milligram of microsomal protein were detected by spectral measurements in microsomes prepared from endosperm tissue of immature Marah macrocarpus seeds. TPNH-cytochrome c reductase, DPNH-cytochrome c reductase, andDPNH-cytochrome b(5) reductase activities were also present in these microsomes at levels of...
The electron-transfer reactions of cellobiose oxidase (CBO) have been investigated by conventional and by rapid-scan stopped-flow spectroscopy at pH 6.0. Analysis of the absorbance/time/wavelength matrix by Singular Value Decomposition (SVD) confirms earlier studies showing that cellobiose rapidly reduces the flavin group (7.7 s-1; cellobiose, 100 microM) which in turn slowly (0.2 s-1) reduces ...
The specific activity and content of cytochrome oxidase in the rough endoplasmic reticulum--mitochondrion complex are higher than in the mitochondrial fraction. Radiolabelling studies with the use of hepatocytes and isolated microsomal and rough endoplasmic reticulum--mitochondrion fractions, followed by immunoprecipitation with anti-(cytochrome oxidase) antibody, reveal that the nuclear-coded ...
It was shown recently that the poky strain of Neurospora accumulates large amounts of cytochrome c, but is deficient in cytochromes a and b and thus in cytochrome oxidase and succinic acid oxidase activities (1). These findings, which suggest that respiration in poky does not utilize the cytochrome system, led to a study of the respiratory system of poky compared with that of wild type. The exp...
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