SPECIFICITY 111 a qualitative test for glycine based on the color developed with pyridine and alkyl chloroformate was recently reported by Sublett and Jewell (13). Except for acetyl glycine and glycine hydrochloride, other amino acids and some other organic structures did not react in their method. In our investigations of the reaction specificity for glycine, it was confirmed that, except for ...

The demethylation of the algal osmolyte dimethylsulfoniopropionate (DMSP) to methylthiopropionate (MTPA) by (homo)acetogenic bacteria was studied. Five Eubacterium limosum strains (including the type strain), Sporomusa ovata DSM 2662(T), Sporomusa sphaeroides DSM 2875(T), and Acetobacterium woodii DSM 1030(T) were shown to demethylate DMSP stoichiometrically to MTPA. The (homo)acetogenic fermen...

The level of fat in the livers of young rats fed a 9 per cent casein-sucrose diet containing choline and the other known lipotropic agents is markedly decreased when additional threonine (0.18 to 0.36 per cent) is included in the diet (1, 2). Harper et al. (3) also found that the deposition of liver fat was reduced when the low protein diet was supplemented with a high level (1 to 1.5 per cent)...

1. Absorption of [3H]glycine and [14C]-glycyl-L-proline at concentrations between 0-5 and 4 mmol/l was studied by perfusion in vivo of rat jejunal and ileal segments. Absorption was defined as net removal of radioisotope from the perfusate. Radioactivity assays and amino acid analyses were performed on perfusates and on mucosal tissue samples obtained from the perfused segments. 2. At the conce...

Activation of N-methyl-D-aspartate (NMDA) receptors is known to produce arachidonic acid release, which has been implicated in excitotoxicity. Antagonists and partial agonists at the glycine site of the NMDA receptor, despite exhibiting functional differences in electrophysiological studies, inhibit glutamate-induced neurotoxicity and ischemia-induced neurodegeneration. The objective of this st...

Glycine transporter 1 (GlyT1) plays a crucial role in regulating extracellular glycine concentrations and might thereby constitute a new drug target for the modulation of glycinergic inhibition in pain signaling. Consistent with this view, inhibition of GlyT1 has been found to induce antinociceptive effects in various animal pain models. We have shown previously that the lidocaine metabolite N-...

L-glycine and L-cysteine, exhibit strong inhibition of partial purified PPO of wild pear. The concentration of L-glycine inhibiting PPO activity by 50% (IC ) was 0.8 and 0.5 mM for catechol oxidation and 50 0.75 and 0.5 mM for pyrogallol oxidation at pH 5 and 7, respectively. IC for L-cysteine calculated, 0.6 and 0.35 50 for catechol oxidation and 0.55 and 0.75 mM for pyrogallol oxidation at pH...

Glycine encephalopathy (GCE) is an autosomal recessive error of glycine degradation, resulting in a poor outcome with severe mental retardation, intractable seizures and spasticity. Milder variants with a significantly better outcome have been reported, but an early prediction of the long-term outcome is not yet possible. With regard to the long-term outcome, the data reported in the literature...

Thiamin, pyridoxin, niacin, and glycine have all been reported to act as growth factors for excised tomato roots grown in sterile culture. White (9) showed that, in basal medium plus thiamin, partial replacement of pyridoxin by glycine could be effected using any one of three different clones of excised roots. The degree to which glycine replaced pvridoxin varied with the different clones. Furt...

Cysteine-rich protein 1 (CRP1) has a unique structure with two well separated LIM domains, each followed by a glycine-rich region. Although CRP1 has been shown to interact with actin-binding proteins and actin filaments, the mechanism regulating localization to the actin cytoskeleton in cells is not clear. Experiments using truncated forms showed that the first LIM domain and glycine-rich regio...