We have developed and optimized a stopped-flow fluorescence assay for use in studying DNA unwinding catalyzed by Escherichia coli RecBCD helicase. This assay monitors changes in fluorescence resonance energy transfer (FRET) between a pair of fluorescent probes (Cy3 donor and Cy5 acceptor) placed on opposite sides of a nick in duplex DNA. As such, this is an "all-or-none" DNA unwinding assay. Si...

A method is described for preparing site-specific mutants using a polymerase chain reaction (PCR) based protocol. The protocol requires a single mutant primer, and has been used to introduce mutations into DNA fragments ranging in size from 200 bp to 1569 bp in length in the GM-CSF, beta-actin, human growth hormone and erythropoietin genes. Sequence analysis of PCR derived mutant fragments show...

This paper describes the evolution of a sector southern coastal plain Pelotas Basin, Brazil, between last Pleistocene sea-level lowstand and Holocene highstand, based on geomorphology high-resolution stratigraphy. A fluvial drainage system associated with an incised valley developed during Last Glacial Maximum (LGM) was identified. Two 2D 2.5D geophysical profiles using Ground-Penetrating Radar...

We evaluated the influence of maternal pre-pregnancy body mass index (BMI), based on reported pre-pregnancy weight and height, on blood pressure (BP) levels during pregnancy by using information from a prospective cohort of 1733 women recruited before 20 weeks' gestation. Maternal antenatal BP values were abstracted from medical records, and we evaluated the mean BP differences according to BMI...

two hundred cases of renal transplantation from living donors are reported from shahid hashemi nejad medical'center in iran. there were 127 male and 73 female patients with 67% in the third and fourth decade of life. all patients received grafts from living donors with 1 14 cases of hla haploidentical, 55 cases of hla identical matches and 24 unrelated donors. one year patient and graft su...

  Introduction Identification of the climate changes is important for understanding the paleoenvironmental characteristics. Sedimentary evidence of the closed basins can be one of the most important methods for identifying the climatic periods and changes in the sedimentary environments. Gavkhouni Playa, as the lowland of the ZayandehRud River catchment area is located in an inter-mountainous...

background: leishmaniasis is a zoonotic disease caused by species of protozoa of the genus leishmania . in recent years, incidence of cutaneous leishmaniasis has increasing trend in golestan province, north of iran. the aim of the present study was to identify the frequency of cutaneous leishmaniasis using pcr-rflp in patients referred to kalaleh health center, during 2013-14. methods: this des...

Newly described outcrops, excavations and sediment cores from the region of Última Esperanza, Magallanes, contain tephra derived from the large late-glacial explosive R1 eruption of the Reclús volcano in the Andean Austral Volcanic Zone. New radiocarbon dates associated to these deposits refine previous estimates of the age, to 14.9 cal kyrs BP (12,670±240 14C yrs BP), and volume, to >5 km3, of...

Macrobrachium olfersii is an amphidromous freshwater prawn, widespread along the eastern coasts of the Americas. This species shows great morphological modifications during ontogenesis, and several studies have verified the existence of a wide intraspecific variation. Because of this condition, the species is often misidentified, and several synonyms have been documented. To elucidate these asp...