Modified nucleosides have the potential to inhibit DNA polymerases for the treatment of viral infections and cancer. With the hope of developing potent drug candidates by the modification of the 2',4'-position of the ribose with the inclusion of a bridge, efforts were focused on the inhibition of Taq DNA polymerase using quantitative real time PCR, and the results revealed the significant inhib...

A multiplex PCR (mPCR) assay was developed for the detection of multiple Salmonella serotypes in different kind of food products. To increase specificity of this molecular method, three sets of oligonucleotide primer were used to detect the most prevalent salmonella species. PhoP primers specific to the PhoP/PhoQ loci of coliform pathogenic bacteria such as Salmonella, Escherichia coli and citr...

Genetic variation in human genome is an emerging resource for studying cancer, a complex set of disease characterized by both environmental and genetic contributions. DNA sequences of the human genome reveal that many genes are polymorphic. In coding or noncoding regions of a specific gene, there may be either a single base pair substitution of one nucleotide (SNPs) for another or a variable nu...

A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction in approximately 2.5 hours . In order to shorten the process with reliable results, several DNA polymerases were tested with the multiplex. Phusion® Flash High Fidelity and Platinum® Taq DNA polymerases were inv...

Abstract Background The high prevalence of low bone mass in young women Japan has emerged as a serious health issue recent years. Therefore, the aim present study was to reevaluate relationship between genetic and dietary factors, well its influence on Japanese women, with particular emphasis vitamin D receptor (VDR) gene polymorphisms calcium intake. Methods A total 499 aged 20–24 years were e...

BACKGROUND Anemia and lead exposure remain significant public health issues in many parts of the world, often occurring together. Animal studies suggest that the dopamine D2 receptor (DRD2) mediates the effects of both lead and iron on cognition and behavior. OBJECTIVE We tested the hypothesis that the DRD2 Taq IA polymorphism modifies the effects of lead and hemoglobin on intelligence quotie...