Chemicals All the oligonucleotides were purchased from Beijing AuGCT DNA-SYN Biotechnology Co. Ltd (Beijing, China). Exonuclease III (Exo III) along with the reaction buffer, 10 × NEBuffer 1 (100 mM bis-tris-propane-HCl, 100 mM MgCl 2 and 10.0 mM dithiothreitol, pH 7.0) and 10 × NEBuffer 3 (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl 2 , 1 mM dithiothreitol pH 7.9) was purchased from New England Bi...

to study the effect of different concentrations of potassium in thenutrient solution and water stress on the quality and quantity yield of lilium la cv.termoli, a pot experiment was conducted based on completely randomized design in sand and perlite medium (50:50) in three levels of potassium (kfree, 6 mm k and and 12 mm k in hoagland solution) with three replications. in the present study, the...

The present report demonstrates a clinical procedure to restore an edentulous area with severe bone destruction using ridge augmentation and implant placement. Titanium-reinforced, expanded polytetrafluoroethylene (ePTFE) membrane and tenting screws combined with a mixture of autogra and allogra (rate 50%: 50%) were used for guided bone regeneration (GBR). A two-stage surgery was performed to r...

OBJECTIVE Multiple myeloma (MM) is characterized by the accumulation and proliferation of malignant plasma cells, secreting monoclonal immunoglobulins and genetic abnormalities in MM have implications for disease progression and survival. In the present study, we investigated the frequency of chromosomal abnormalities (CA) in Turkish patients with MM, using interphase FISH and CC and evaluated ...

Canine cardiac Purkinje fibers exposed to sodium-free solutions containing 16 mM CaCl2, 20 mM tetraethylammonium chloride, 108 mM tetramethylammonium chloride, and 2.7 mM KCl may be quiescent at a resting potential of either -50 mV or -90 mV. The membrane potential of these fibers can be switched from -50 mV to -90 mV by a hyperpolarizing current pulse and from -90 mV to -50 mV by a depolarizin...

BACKGROUND In patients with idiopathic dilated cardiomyopathy (IDCM), the myocardial blood flow is markedly depressed. The presence of alterations in the number and length of the coronary microcirculation has not been explored. METHODS In explanted hearts of 6 patients with IDCM and in 6 normal control hearts, the arteriolar length density and capillary numerical density were determined in se...

This study was undertaken to evaluate the efficacy of a new low-temperature sterilization system that recently has been cleared by the Food and Drug Administration, the Sterrad 50. Flat stainless steel carriers were inoculated with approximately 10(6) Bacillus stearothermophilus spores. These carriers were placed aseptically in the middle of 40-cm-long stainless steel-lumened test units of vary...

       The oxidative hemolysis of rat erythrocytes induced by 2,2’-azobis–(2-amidinopropane) (AAPH) and its inhibition by rosemary essential oil was studied. Different concentrations (0.178, 0.357, 0.534 and 0.712 ml/ml) of the essential oil showed no significant hemolysis compared to phosphate buffer solution. AAPH (25 mM and 50 mM) induced hemolysis in a time-dependent manner. Diff e r e n t ...