Different chemotypes are described as anti-inflammatory. Among them the N-acylhydrazones (NAH) are highlighted by their privileged structure nature, being present in several anti-inflammatory drug-candidates. In this paper a series of functionalized 3-aminothiophene-2-acylhydrazone derivatives 5a-i were designed, synthesized and bioassayed. These new derivatives showed great anti-inflammatory a...

Two variants of the human myeloid leukemia cell line HL-60 were used to study the possible involvement of the IFN-induced protein 2-5A synthetase in cell growth arrest and differentiation. The two variants, HL-205 and HL-525, are equally susceptible to differentiation to the granulocyte lineage by exposure to DMSO, but only HL-205 cells acquire the macrophage phenotype following exposure to pho...

This paper provides an overview of the evidence addressing the impairments of the 2'-5' oligoadenylate (2-5 A) synthetase/RNase L pathway in Chronic Fatigue Syndrome (CFS) patients. The 2-5A synthetase/RNase L pathway in CFS patients appears to be both up-regulated (i.e. increased levels of bioactive 2-5A synthetase and increased activity of the RNase L enzyme) and deregulated (elastase and cal...

To define protein domains important for activation of the interferon (IFN)-induced enzyme 2-5A-dependent RNaseL, we have generated vaccinia virus (VV) recombinants able to express in cultured cells truncated forms of this protein and compared their biologic activities with those producing the wild-type enzyme, with and without coexpression of 2-5A synthetase. Our results show that full activati...

Introduction: Myocardial infarction (MI) is a multifactorial disease influenced by environmental and genetic factors. Matrix metallo proteinase-3 (MMPs) plays a pivotal role in the development of atherosclerosis and MI. Objective: The objective of the present study was designed to investigate the association of matrix metallo proteinase3 -1612 promoter 5A/6A gene polymorphism. Methods: In the p...

In vivo responses to interferon (IFN) in mice were determined by measuring the steady-state levels of induced mRNAs following injection of IFN and poly(I)-poly(C). With cDNA probes for mouse 2'-5' oligoadenylate synthetase (2-5A synthetase) and 1-8, constitutive expression of the corresponding mRNA was detectable in different organs of normal C3H/He mice. These mRNA levels were increased by as ...

UNLABELLED Synthesis of 2'-5'-oligoadenylates (2-5A) by oligoadenylate synthetase (OAS) is an important innate cellular response that limits viral replication by activating the latent cellular RNase, RNase L, to degrade single-stranded RNA. Some rotaviruses and coronaviruses antagonize the OAS/RNase L pathway through the activity of an encoded 2H phosphoesterase domain that cleaves 2-5A. These ...

The chemistry of white phosphorus has generated much interest over the past decades owing to its unique tetrahedral structure and variable bonding situation. Organophosphorus compounds can be used as reagents and as ligands for innumerable complexes in catalytic processes. The reactions of transition-metal complexes with white phosphorus have been extensively studied and have resulted in a larg...

conclusions this study provided conserved b-cell epitopes and peptides that can be useful for designing entry inhibitors and vaccines able to cover a global population, especially where genotype 5a is common. results differences in the probability of glycosylation in e1 and e2 regions were observed in this study. three conserved antigenic b-cell epitopes were predicted in the e2 regions and als...

Reaction of methyl undec-10-enoate 2 with cyclopentanone 1a affords two products, methyl 2-(2′-cyclopentanonyl)undec-10-enoate 3a and methyl 11-(2′-cyclopentanonyl)undecanoate 4a. Similarly, the reaction of cyclohexanone 1b gives two products 3b and 4b. Compounds 4a and 4b on further reaction with mercaptoacetic acid and ammonium carbonate yield the products 5a and 5b, respectively. Products ha...