RNA, extracted from peripheral blood mononuclear cells (PBMC) obtained from 81 Korean psychiatric patients (39 with schizophrenia, 33 with bipolar affective disorders and nine with major depression), was analyzed for a 391-nucleotide, highly conserved region of the p24 protein-encoding ORF II of Borna disease virus (BDV), using nested reverse transcription-polymerase chain reaction (RT-PCR). BD...

Free-roaming and scavenging lifestyles of Rattus spp. in densely populated urban areas expose them to multiple pathogens that facilitate the transmission infection human population more rapidly, raising public health concerns. There is limited information on status rat susceptibility virus infection, particularly West Nile (WNV), herpesvirus, parvovirus, prepare for emerging zoonosis. A total 2...

BACKGROUND Occult hepatitis C virus (HCV) infection is a new entity described by the presence of HCV-RNA in liver biopsy and/or peripheral blood mononuclear cell (PBMC) specimens, and undetectable levels or absence of HCV-RNA and in the absence or presence of anti HCV antibodies in plasma by current laboratory methods. OBJECTIVES To evaluate the detection of HCV-RNA in PBMC specimens of the l...

Background: Fast and precise detection of SARS-CoV-2 RNA in clinical samples subsequent quarantine are two critical factors preventing virus transmission distribution through the community. The false-negative result is a major problem because kind sample (swab sample), sampling error, sensitivity PCR test, which can be reduced by much more sensitive test such as nested PCR. Objectives: This stu...

BACKGROUND Feline infectious peritonitis is a fatal disease of cats caused by infection with feline coronavirus (FCoV). For detecting or genotyping of FCoV, some RT-PCR plus nested PCR techniques have been reported previously. However, referring to the whole genome sequences (WGSs) registered at NCBI, there are no detection methods that can tolerate the genetic diversity among FCoV population. ...

We aimed to investigate whether the sequence length of HIV-1 increases over time. performed a longitudinal analysis full-length coding region sequences (FLs) during an outbreak among patients with hemophilia and local controls infected Korean subclade B (KSB). Genes were amplified by overlapping RT-PCR or nested PCR subjected direct sequencing. Overall, 141 FLs sequentially determined 30 years ...

PURPOSE We present the largest study of both peripheral blood and lymph node samples examining the utility of reverse transcription-polymerase chain reaction (RT-PCR) for established molecular markers as a diagnostic tool in the molecular staging of prostate cancer patients undergoing radical prostatectomy. EXPERIMENTAL DESIGN Peripheral blood from 358 patients was obtained before radical pro...

Non-specific tail sequences are often added to the 5'-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in vitro and in silico analysis of the enhancing effect of primer tailing on 2 well-established foo...

Noroviruses (NoV), which are members of the family Caliciviridae, are the most important cause of outbreaks of acute gastroenteritis worldwide and are commonly found in shellfish grown in polluted waters. In the present study, we developed broadly reactive one-step TaqMan reverse transcription (RT)-PCR assays for the detection of genogroup I (GI) and GII NoV in fecal samples, as well as shellfi...

To test whether patients in remission after allogeneic bone marrow transplantation (BMT) possess a pool of chronic myeloid leukemia (CML) cells that do not express BCR-ABL mRNA, we have compared the results and sensitivity of amplification of BCR-ABL from genomic DNA with conventional reverse transcription-polymerase chain reaction (RT-PCR). Bubble PCR was used to amplify the genomic BCR-ABL tr...