Clinacanthus nutans (Burm. f.) Lindau is a medicinal plant rich in polyphenols and possesses multiple pharmacological properties. It widely used as side treatment for cancer to prevent oxidative stress-related diseases. The present study aims investigate the effects of light illumination subculture frequency on growth proliferation cells suspension culture C. nutans. Friable callus induced from...

Lilium brownii var. viridulum, commonly called Longya lily, is a well-known flower and vegetable plant in China that has poor tolerance to Botrytis fungal disease. The molecularimprovement mainly been restricted an efficient regeneration transformation system. In this study, the highly of lily was established through optimization embryogenic callus, adventitious shoot rooting induction. major f...

Biotechnological approaches provide an efficient and expeditious system for developing agronomic features of rice at the cellular molecular level compared to traditional breeding methods. The in-vitro regeneration method optimization recalcitrant indica genotypes is a critical step biotechnological further development as optimum concentrations Plant Growth Regulators (PGRs) vary from genotype g...

Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine species are Yunnan with most occurring South America, making P. highly significant emblematic the conservation work region. This study designed provide first protocol for vitro organogenesis plant regeneration ex situ ...

ABSTRACT: Effective protocols for in vitro rooting woody fruit trees are still a challenge seedling production, especially when there is need to insert new cultivars or rootstocks. These essential accelerate studies plant breeding programs and distribution. This study evaluated the use of 6-Benzylaminopurine (IBA) Pyruscomunnis rootstocks, clones ‘OHxF87’ Pyrodwarf. Explant exposure times (0, 2...

Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation and environment disturbance. This study was conducted to provide propagation methods through in vitro germination explant cultivation. Germination stimulated up 66.00% on Murashige Skoog (MS) medium containing 2.0 mg L−1 6-benzylaminopurine (BAP), 0.1 1-napthaleneacetic acid (NAA), 30...

Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were o...

The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal m...

A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induc...

Hormonal and plant factors regulating the development of the inhibited cotyledonary buds of Lycopersicon esculentum Mill. cv. ;Fireball' seedlings were studied. Excision of the immature plumular leaves of 5- to 20- millimeter length significantly stimulated bud development after 2 to 4 days, but excision of leaves exceeding 20-millimeter length was without effect. Apical application of 20 micro...