many studies have proved that the dietary micronutrient has an inhibitory effect against experimentally induced rat hepatocarcinogenesis. the present work is an attempt to understand combined effect of selenium (se) and vitamin d3 (vit d3) on some potential protein expression markers of carcinogenesis, such as metallothionein (mt), p53 and antioxidant levels during diethyl nitrosamine (den) ind...

Metallothionein (MT) is important because it binds tightly to heavy metals to decrease their toxicity. DNA was isolated from 30 toxic metal exposed and 30 toxic metal unexposed Zebu cows. The amplified metallothionein isoform-2 (MT-2) PCR product (489 bp) was further used for PCR-RFLP and DNA sequencing. MT-2 TaqI PCR-RFLP revealed homozygous genotype (AA) except for the E23 animal (AB). The ge...

A rapid culture assay which allows for the simultaneous typing and subtyping of currently circulating influenza A(H1N1), A(H3N2), and B viruses in clinical specimens was developed. Pools of monoclonal antibodies (MAbs) against influenza A and B viruses and MAbs HA1-71 and HA2-76, obtained by immunizing mice with the denatured hemagglutinin subfragments HA1 and HA2 of influenza virus A/Victoria/...

Previous studies using a cardiac-specific metallothionein (MT)-overexpressing transgenic (MT-TG) mouse model have demonstrated that MT protects from doxorubicin (DOX)-induced oxidative heart injury. The molecular mechanisms that underlie this cardioprotection, however, have yet to be defined. In the present study, we tested the hypothesis that MT overexpression activates cytoprotective mechanis...

To understand mechanisms for arsenic toxicity in the lung, we examined effects of sodium m-arsenite (As³⁺) on microtubule (MT) assembly in vitro (0-40 µM), in cultured rat lung fibroblasts (RFL6, 0-20 µM for 24 h) and in the rat animal model (intratracheal instillation of 2.02 mg As/kg body weight, once a week for 5 weeks). As³⁺ induced a dose-dependent disassembly of cellular MTs and enhanceme...

       Many studies have proved that the dietary micronutrient has an inhibitory effect against experimentally induced rat hepatocarcinogenesis. The present work is an attempt to understand combined effect of selenium (Se) and vitamin D3 (vit D3) on some potential protein expression markers of carcinogenesis, such as metallothionein (MT), P53 and antioxidant levels during ...

MOTIVATION Worldwide and substantial mortality caused by the 2009 H1N1 influenza A has stimulated a new surge of research on H1N1 viruses. An epitope conservation has been learned in the HA1 protein that allows antibodies to cross-neutralize both 1918 and 2009 H1N1. However, few works have thoroughly studied the binding hot spots in those two antigen-antibody interfaces which are responsible fo...

Two experiments were conducted to study the efficacy of a low inclusion commercialHSCAS (Myco-Ad) in preventing the deleterious effects of T-2 toxin (T-2) in broilerchicks. Feed in both experiments was experimentally contaminated with synthetic T-2from Sigma Labs, USA. In Experiment 1, 75 1-d-old Arbor Acres straight-run broilersindividually caged were randomly distributed into ...