Phytate is the major storage form of organic phosphorus in soils and plant seeds, and phosphorus (P) in this form is unavailable to plants or monogastric animals. In the present study, the phytase genes phyA and appA were introduced into Brassica napus cv Westar with a signal peptide sequence and CaMV 35S promoter, respectively. Three independent transgenic lines, P3 and P11 from phyA and a18 f...

From the last few years, the inclusion of microbial phytase in poultry diets has increased significantly, mainly in response to heightened concerns over phosphorus pollution of the environment and as cheaper means to make phosphorus available to birds from phytate. Phytate is the major form of phosphorus, abundantly found in cereal grains, beans and oilseed meals used in poultry diet but the mo...

The nitro group of 2-nitroimidazole (NIM) enters the tumor cells and is bioreductively activated and fixed in the hypoxia cells. 1,4,8,11-tetraazacyclotetradecane (N4) has shown to be a stable chelator for (99m)Tc. The present study was aimed to develop (99m)Tc-cyclam-2-nitroimidazole ((99m)Tc-N4-NIM) for tumor hypoxia imaging. N4-NIM precursor was synthesized by reacting N4-oxalate and 1,3-dib...

Iron is a vital nutrient to promote the availability of tissue oxygen, cell growth and control differentiation, energy metabolism. Preventing Deficiency Anemia (IDA) necessary because iron central nervous system development especially in first years life. Iron-rich complementary foods are recommended infants around 6 months age store depleted. Better understanding absorption process factors aff...

The current study was undertaken to estimate variance components for phytate P bioavailability (PBA) and the genetic correlations among PBA with growth and feed utilization (or intake) traits in an unselected random mating chicken population. Pedigreed data from 901 Athens-Canadian randombred chickens hatched from 26 sires, 71 dams, and 105 grandparents were used for estimation of genetic param...

The capacity of wheat bran to impair mineral absorption independent of its phytate content was studied by metabolic balance studies in man. Three breads were prepared, equivalent to white, brown and wholemeal, by adding bran in different quantities to white flour. Calcium, iron, zinc and sodium phytate were added to the loaves to make the amounts equal in all breads. Six healthy volunteers were...

An Aspergillus niger UFV-1 phytase was characterized and made available for industrial application. The enzyme was purified via ultrafiltration followed by acid precipitation, ion exchange and gel filtration chromatography. This protein exhibited a molecular mass of 161 kDa in gel filtration and 81 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), indicating that it m...

(99m)Tc-HYNIC-annexin A5 can be considered as a benchmark in the field of apoptosis imaging. However, (99m)Tc-HYNIC-annexin A5 has characteristics of high uptake and long retention in non-target tissues such as kidney and liver. To minimize this problem, we developed a novel (99m)Tc-labeled annexin A5 using a bis(hydroxamamide) derivative [C3(BHam)2] as a bifunctional chelating agent, and evalu...

A reliable geochemical paleo-indicator for phosphorus remains elusive, despite the importance of understanding historical changes in the nutrient status of aquatic ecosystems. We assessed the potential of phytate (salts of mvo-inositol hexakisphosphate) as a novel phosphorus-specific paleoindicator by measuring its concentrations in dated sediments from an embayment in Helsinki, Finland, with a...

Phytases are enzymes capable of sequentially dephosphorylating phytic acid to products of lower chelating capacity and higher solubility, abolishing its inhibitory effect on intestinal mineral absorption. Genetic constructions were made for expressing two phytases from bifidobacteria in Lactobacillus casei under the control of a nisin-inducible promoter. L. casei was able of producing, exportin...