Abstract To understand the potential effects of cancer cells on surrounding normal mammary epithelial cells, we performed direct co-culture non-tumorigenic MCF10A and various breast cells. Firstly, observed dynamic cell–cell interactions between including lamellipodia or nanotube-like contacts transfer extracellular vesicles. Co-cultured exhibited features epithelial-mesenchymal transition, sho...

Abstract Over application of nitrogen (N) fertilizers to crops ultimately causes N pollution in the ecosphere. Studying response plant growth and uptake low-N stress may aid elucidating mechanism low tolerance plants developing crop cultivars with high use efficiency (NUE). In this study, a high-NUE mutant line A9-29 wild-type barley cultivar Hua30 were subjected hydroponic culture supply, dry ...

Introduction Calprotectin (S100A8/A9) is an example of a damage associated molecular pattern and a TLR4 ligand. It is expressed in neutrophils, monocytes and early infiltrating macrophages. Calprotectin, once secreted, has a number of proinflammatory effects on activated endothelium and on phagocytes and reflects activation of the innate immune system. Calprotectin has been demonstrated to be s...

We have characterized a novel Zn2+-catalyzed cleavage site between nucleotides C3 and U4 in the catalytic core of the hammerhead ribozyme. In contrast to previously described divalent metal-ion-dependent cleavage of RNA, U4 cleavage is only observed in the presence of Zn2+. This new cleavage site has an unusual pH dependence, in that U4 cleavage products are only observed above pH 7.9 and reach...

INFLAMMATORY MEDIATORS TRIGGER POLYMORPHONUCLEAR NEUTROPHILS (PMN) TO PRODUCE REACTIVE OXYGEN SPECIES (ROS: O(2) (-), H(2)O(2), ∙OH). Mediated by myeloperoxidase in PMN, HOCl is formed, detectable in a chemiluminescence (CL) assay. We have shown that the abundant cytosolic PMN protein calprotectin (S100A8/A9) similarly elicits CL in response to H(2)O(2) in a cell-free system. Myeloperoxidase an...

Secretion of inflammatory products from neutrophils can be induced by a combination of signals from ligated integrins and receptors for soluble, physiological agonists such as TNF. Here we identify pyk2 in primary human neutrophils; localize it to focal adhesions and podosomes; and demonstrate its tyrosine phosphorylation, activation, and association with paxillin during stimulation of adherent...

s directly related to the thesis 1. Bodosi B, Gardi J, Hajdu I, Szentirmai É, Obál F Jr., Krueger JM. Ghrelin and leptin responses to sleep deprivation and to reversed feeding schedule in rats. J Sleep Res 13, S1, p. 83, 2004. 2. Gardi J, Bodosi B, Hajdu I, Szentirmai É, Obál F Jr., Krueger JM. Rhythms of ghrelin and leptin with respect to sleep and feeding in rats. J Sleep Res 13, S1, p. 259, ...

Dimerization of genomic RNA is directly related with the event of encapsidation and maturation of the virion. The initiating sequence of the dimerization is a short autocomplementary region in the hairpin loop SL1. We describe here a new solution structure of the RNA dimerization initiation site (DIS) of HIV-1(Lai). NMR pulsed field-gradient spin-echo techniques and multidimensional heteronucle...