Activation of plasma kallikrein arginine esterase activity by kaolin resulted in peak activity at 1 min of incubation and a 50% reduction in activity at 5 min in normal plasma, and 30% reduction in the plasma of patients with hereditary angioneurotic edema who lacked the C1 inactivator. The peak esterolytic activity was inhibited by soybean trypsin inhibitor whereas the 5 min activity was resis...

A megakaryoblastic cell line, termed T-33, was established from the peripheral blood of a patient with Philadelphia chromosome-positive chronic myelogenous leukemia in megakaryoblastic crisis. T-33 cells have been maintained in RPMI 1640 medium containing 10% fetal calf serum in a single cell suspension with a doubling time of 24-36 h for over 2 years. Giemsa-banded karyotypes were female hyper...

We have investigated the kinetic properties of acid cholesteryl esterase (EC 3.1.1.13) in preparations of rabbit aortic cells, with the aim of establishing conditions suitable for the quantitative assay of the enzyme in freshly prepared homogenates and subcellular fractions, whether derived from normal cells or from atheromatous cells heavily laden with cholesterol and cholesteryl esters. As su...

In this work, genome mining was used to identify esterase/lipase genes in the archaeon Pyrobaculum sp. 1860. A gene was cloned and functionally expressed in Escherichia coli as His-tagged protein. The recombinant enzyme (rP186_1588) was verified by western blotting and peptide mass fingerprinting. Biochemical characterization revealed that rP186_1588 exhibited optimum activity at pH 9.0 and 80 ...

The 3BM-78 murine Friend erythroleukemia cell line was obtained by in vitro transformation of bone marrow cells of DBA/2J mice by the polycythemic Friend virus complex. Thirty-five subclones have been isolated and tested for their ability to express various markers of blood and bone marrow cells. Upon dimethyl sulfoxide treatment, the cells differentiated along the erythroid pathway as shown by...

Prof. Dr. D. Huhn, Medizinische Klinik III, Klinikum Grosshadern, Universität München, Marchioninistr.15, D-8000 München 70 (FRG) In this journal, Veerman et al. [6] claimed that the vast majority of T-ALL cells proved to be ANAE negative (30/31) and acid phosphatase positive (29/31). As ANAE positivity in ALL largely depends on the fixation used [1,5] this point will be emphasized in the follo...

The carmine spider mite, Tetranychus cinnabarinus is an important pest of crops and vegetables worldwide, and it has the ability to develop resistance against acaricides rapidly. Our previous study identified an esterase gene (designated TCE2) over-expressed in resistant mites. To investigate this gene's function in resistance, the expression levels of TCE2 in susceptible, abamectin-, fenpropat...

Nonspecific esterase (NSE) is used to identify normal and leukemic mononuclear phagocytes cytochemically. It can be demonstrated by the hydrolysis of alpha-naphthyl acetate or butyrate. NSE from leukapheresed leukemic monocytes were extracted with several types of detergent and grouped into two isoelectric point (pl) categories based on the ease of solubility: pl 5.7-6.3 (8 bands) greater than ...

biomarkers are assigned as early warning systems regarding aquatic organisms exposed to pollutants. among which, however, measurement of esterase enzymes activity specially cholinesterase of fish tissues, is a marker for exposure to organophosphate pesticide and carbamates, and this biomarker has been used widely in broad range of investigations. a number of capoeta capoeta gracilis (:cyprinida...

As long ago as 1905, Warburg (1) employed the amino acid esterase activity of tissues to prepare the optical isomers of amino acids. Since that time, other than for the studies of esterase activity of the proteases, there have been only scattered reports of the amino acid esterase activity of tissue extracts. In fact, Smith and coworkers (24) have emphasized that their preparations of “leucine ...