The ketosidic linkage of 3-deoxy-d-manno-octulosonic acid (Kdo) to lipid A constitutes a general structural feature of the bacterial lipopolysaccharide core. Glycosylation reactions of Kdo donors, however, are challenging due to the absence of a directing group at C-3 and elimination reactions resulting in low yields and anomeric selectivities of the glycosides. While 3-iodo-Kdo fluoride donors...

The pattern of growth of aerial mycelium in Streptomyces species was investigated by autoradiography. Colonies of Streptomyces antibiotics were labeled with N-acetyl-D-[1-3H] glucosamine to localize the sites of hyphal growth during the development of aerial mycelium. Autoradiographs obtained with sections of the colonies revealed that hyphal growth occurs not only at the top of the colony but ...

The growth of corn (Zea mays) roots and barley (Hordeum vulgare) coleoptiles is sensitive to the presence of external d-glucosamine and d-galactose. In order to investigate this effect, tissues were fed the radioactive monosaccharides at concentrations that ranged from those that were strongly inhibitory to those that had little influence on growth. At low concentrations, d-glucosamine is conve...

d-Glucosamine-6-P N-acetyltransferase (EC 2.3.1.4) from mung bean seeds (Phaseolus aureus) was purified 313-fold by protamine sulfate and isoelectric precipitation, ammonium sulfate and acetone fractionation, and CM Sephadex column chromatography. The partially purified enzyme was highly specific for d-glucosamine-6-P. Neither d-glucosamine nor d-galactosamine could replace this substrate. The ...

Chitin is a universal biopolymer that found in microbes, plants, fungi, the exoskeleton of insects, various species algae, and bottom-feeding crustaceans. This (1–4)-linked N-acetyl-ß-D-glucosamine polysaccharide can be readily processed with simple chemical procedures without putting at risk. has garnered interest as an alternative substance used medical, environmental, agricultural sectors. I...

A general single-step approach is introduced for the green fabrication of hybrid biosensors from water dispersion. The resulting device integrates semiconducting properties a carbon nanotube (CNT) and functionality protein. In initial aqueous phase, protein (viz., lysozyme [LZ]) disperses (6,5)CNT. Drop-casting dispersion on test pattern (a silicon wafer with interdigitated Au source drain elec...

Shrimp waste is a perishable material. The degradation process carried out by decaying microbes producing degrading enzymes. presence of chitin content in shrimp causes the emergence chitinase enzyme-producing bacteria. Chitin linear homopolymer composed N-acetyl-D-glucosamine monomers with -(1,4) glycosidic bonds. has unique properties, namely; biodegradable, biocompatible and non-toxic. This ...

BACKGROUND Glucosamine 6-phosphate deaminase from Escherichia coli is an allosteric hexameric enzyme which catalyzes the reversible conversion of D-glucosamine 6-phosphate into D-fructose 6-phosphate and ammonium ion and is activated by N-acetyl-D-glucosamine 6-phosphate. Mechanistically, it belongs to the group of aldoseketose isomerases, but its reaction also accomplishes a simultaneous amina...

OBJECTIVE The hexosamine biosynthesis pathway (HBP) flux and protein O-linked N-acetyl-glucosamine (O-GlcNAc) levels have been implicated in mediating the adverse effects of diabetes in the cardiovascular system. Activation of these pathways with glucosamine has been shown to mimic some of the diabetes-induced functional and structural changes in the heart; however, the effect on cardiac metabo...