The results 1 from measurements made with the glass electrode and spectrophotometer show that brilliant cresyl blue penetrates as free base into the vacuoles of living cells of Nitella. The present paper describes similar experiments with azure B, methylene blue, and crystal violet. Since the methods for the condition of cells, extraction of the sap, penetration experiments, extraction of free ...

Trichromatic colorimetry was applied for the evaluation of the colour changes of the metallochromic indicators, viz., hematoxylin, gallocyanine, Gallamine Blue, Solochrome Prune AS, Celestine Blue, Eriochrome Black T, Xylenol Orange and Catechol Violet, in the complexometric titration of zirconium( IV) with ethylenediaminetetraacetic acid (EDTA) to measure the quality of colour change at the eq...

Green luminescent water-soluble citric acid-carbon dots composite were synthesized from empty fruit bunches (EFB) biochar. The hydrothermal method solves the weakness of difficult size to control and large energy. has advantages being easy, environmentally friendly, not requiring energy, particle is easy control. average composites was 4 nm. resulting belongs n-π* transition carbonyl groups C–N...

In a weak acid medium, protein reacts with a monoazo dye of chromotropic acid, such as Chromazol KS (CALKS), Acid Chrome Dark Blue (ACDB), Chrome Blue SE (CBSE), Acid Chrome Blue K (ACBK), Chlorophosphonazo I (CPAI), Arsennazo I (AAI) and Chromotrope 2R (CT2R), to form a combination product. This results in a significant enhancement of the resonance Rayleigh-scattering (RRS) intensity, and the ...

Although protein is the third most abundant solid in bile and is important in cholesterol crystal formation, methods for quantitating the concentration of total protein in bile have not been systematically evaluated. To establish a reliable protein assay for bile, we evaluated three protein assays (Lowry's method and the fluorescamine and Coomassie blue methods), and employed amino acid analysi...

of cancer with the hope of developing useful diag nostic and therapeutics agents. The aim in such investigations has been to find a dye which, in the intact animal, stains living tumor cells without staining surrounding tissue. A large number of acid dyes, especially in the azo series, has been rather intensively investigated for such a purpose. From these investigations there has resulted gen ...

Blue-M1 is a blue pigment formed from xylose and glycine in the Maillard reaction. Previous work revealed that Blue-M1 scavenged hydroxyl radicals, and prevented the autoxidation of linoleic acid in vitro. We investigated the protective effect of Blue-M1 for 2,2'-azobis(2-amidino-propane)dihydrochloride (AAPH)-induced toxicity in COS-1 cells. COS-1 cells were cultured in AAPH containing DMEM me...