Purified 2,4-dichlorophenoxyacetic acid (2,4-D)/(alpha)-ketoglutarate dioxygenase (TfdA) was shown to use 4-nitrophenoxyacetic acid (K(infm) = 0.89 (plusmn) 0.04 mM, k(infcat) [catalytic constant] = 540 (plusmn) 10 min(sup-1)), producing intensely yellow 4-nitrophenol. This reagent was used to develop a rapid, continuous, colorimetric assay for the detection of TfdA and analogous activities in ...

2-amino-5-carboxymuconic 6-semialdehyde is an unstable intermediate in the meta-cleavage pathway of 4-amino-3-hydroxybenzoic acid in Bordetella sp. strain 10d. In vitro, this compound is nonenzymatically converted to 2,5-pyridinedicarboxylic acid. Crude extracts of strain 10d grown on 4-amino-3-hydroxybenzoic acid converted 2-amino-5-carboxymuconic 6-semialdehyde formed from 4-amino-3-hydroxybe...

The nucleotide sequence of -full length leghemoglobin (Lb) cDNA " clone -from yellow lupin (Lupinus luteus) is presented. The amino acid sequence deduced -from the nucleotide sequence is in accor— "* dance with known ammo acid sequence of lupin Lb I e;:cept position 79 where GLn is -found instead o-f GILI. Several conserved * sequence mot i -fs, found also in other Lb genes C13 are underlined "...

Abstract Recently, the adsorption process relied on use of low-cost, available and eco-friendly materials to reduce costs. Pomegranate peels(PPs) have been used as environmental inexpensive bio-sorbent material remove dye (brilliant yellow) from aqueous solution. characterization via two techniques like FTIR, FESEM analyze morphology chemical groups before after sorption brilliant yellow dye, A...

Discrete bodies which may be designated cytochondria occupy the greater part of the cytoplasm of liver cells. A part, but not all, of these bodies have the characteristics of mitochondria. They consist of a rim which stains deeply and a central part which stains faintly or remains unstained. In liver cells about portal spaces, in foci of regeneration, and in tumor cells the rims of the cytochon...

AIM To identify pathogen of diseased yellow perch and determine their virulence. METHODS AND RESULTS Fifteen Gram-negative bacterial isolates were recovered from the skin lesions of diseased yellow perch (Perca flavescens). Based on API 20NE test, ten isolates were found to share 67.2-99.9% homologies with Chryseobactertium indologenes. Based on fatty acid methyl ester analysis, 13 isolates w...

PURPOSE To investigate the molecular mechanism underlying intrinsic resistance to ABT-199. EXPERIMENTAL DESIGN Western blots and real-time RT-PCR were used to determine levels of Mcl-1 after ABT-199 treatment alone or in combination with cytarabine or daunorubicin. Immunoprecipitation of Bim and Mcl-1 were used to determine the effect of ABT-199 treatment on their interactions with Bcl-2 fami...

The antioxidant activity and contents of various polyphenol classes in the seeds of seven soybean varieties of different seed color and one yellow seed cultivar, representing a reference genotype, were evaluated. Total polyphenols and tannins were determined after extraction of plant material with 70% aqueous acetone, and total flavonoids were extracted with methanol and acetic acid, whereas an...