Reports indicate an increase in the incidence of DNA fragmentation in male factor infertility and its role in the outcome of assisted reproductive techniques (ART). However, reports are conflicting between the relationships of sperm DNA integrity with conventional semen parameters. We examined the relationship between conventional sperm parameters and DNA integrity using acridine orange (AO) te...

Hallap T. 2005. Assessment of sperm attributes of frozen-thawed AI doses from Swedish and Estonian dairy bull sires. With special reference to pre-selection through swim-up, and the influence of age on potential fertility. Doctoral thesis ISSN 1652-6880, ISBN 91-576-6912-0 The fertility of bull semen used in artificial insemination (AI) is essential for the effective use of this technology in b...

P-Nonylphenol (p-NP) as a non ionic surfactant is used in many industries such as pesticides, cosmetics, detergents and so on. Our previous study showed that the p-NP causes reduction in viability of the rats Bone Marrow Mesenchymal Stem cells (MSCs). The aim of the present study was to investigate the mechanism of the cell death due to p-NP exposure. The cells, after 3 passage were treated wit...

The aim of this study was to determine the extent of DNA fragmentation and the presence of denatured single-stranded or normal double-stranded DNA in spermatozoa with large nuclear vacuoles (LNV) selected by high magnification. Fresh semen samples from 30 patients were prepared by discontinuous isolate concentration gradient. Spermatozoa with normal nucleus (NN) and LNV were selected at x8400 m...

It is now widely accepted that chromatin is a highly dynamic structure that participates in all DNA-related functions, including transcription, DNA replication, repair, and programmed cell death. Chromatin compaction influences plant regeneration and development. In plants, the most popular method of detecting chromatin compaction is immunofluorescence coupled with confocal microscopy. This met...

in human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. the sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. the objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...