The objective of this study was to determine the optimal cutoff value of serum adenosine deaminase (ADA) activity for diagnosing scrub typhus in acute febrile patients, especially in the autumn. A total of 715 febrile patients were included; 286 of them were diagnosed with acute scrub typhus. The mean serum ADA activities in patients with and without scrub typhus were 42.8 ± 13.4 IU/L and 17.2 ...

Introduction: Tuberculous pleurisy is one of the most common form of extrapulmonary tuberculosis (TB). Measurement of adenosine deaminase (ADA) level in the effusions is advocated to have a high diagnostic yield in the diagnosis of tuberculous pleurisy as well as pericardial and peritoneal effusions due to TB. The diagnostic yield of ADA measurement in other materials such as abscess content ha...

Adenosine deaminase (ADA) activity has been measured in the lymphoblasts of 23 untreated patients with acute lymphoblastic leukemia and related to the presence or absence of immunologic cell surface markers. The mean ADA activity in the acute lymphoblastic leukemia population as a whole was increased fourfold over that in normal lymphocytes. 9 of the 23 patients were classified as thymus-derive...

A high titer retroviral vector was used to transfer a human adenosine deaminase (h-ADA) cDNA into murine bone marrow cells in vitro. The h-ADA cDNA was linked to the retroviral promoter, and the vector also contained a neomycin phosphotransferase gene as a selectable marker. Infected marrow was transplanted into syngeneic W/W’ recipients, and h-ADA expression was monitored for 5.5 months. Sever...

The diagnostic value of adenosine deaminase (ADA) activity was studied to evaluate its use in the differential diagnosis of tuberculous meningitis in the local setting. Cerebrospinal fluid (CSF) from 119 patients with meningitis and other conditions with central nervous system symptoms were collected and ADA activity determined by the colorimetric method of Guisti read at 628 nm. The CSF was al...

Overexpression of adenosine deaminase (ADA) in red blood cells is characterized by a marked, tissue-specific increase in levels of structurally normal ADA mRNA and enzymatic activity in the erythrocytes of affected individuals, leading to adenosine triphosphate (ATP) depletion and hemolytic anemia. This autosomal dominant trait is linked to the ADA gene. To investigate the molecular mechanism r...

The aim of this study is whether oxidative stress markers, homocysteine (Hcy), cardiac troponin I (cTnI) and the activity of adenosine deaminase (ADA) alter following administration of different doses of sertraline. Sixty male Wistar rats were assigned into four groups. Sertraline at doses of 20, 40 and 80 mg / kg / day) were administered to three groups through gastric gavage for 90 days. The ...

Cerebrospinal fluid (CSF) adenosine deaminase (ADA) activity in tubercular meningitis (TBM) patients (n=20), non-tubercular meningitis (NTBM) patients (n=10) and non-tubercular non-meningitis (NTBNM) cases (n=15) were measured by the method based on Berthlots reaction. The mean CSF ADA activity in TBM (13.62 ± 8.45 IU/L) was found to be significantly higher as compared to NTBM (6.51 ± 2.41 IU/L...

Aims: Insulin resistance is consistence feature in type 2 diabetes mellitus (DM). Several studies demonstrated that serum adenosine deaminase activity (ADA) is elevated in type 2 DM patients. The present study was conducted to estimate serum ADA activity in newly diagnosed type 2 DM patients, compared with control groups and to find out correlation of insulin resistance with serum ADA activity....

The biochemical mechanisms whereby an inherited deficiency of adenosine deaminase (ADA; EC 3.5.4.4) activity results in selective toxicity to lymphoid cells has been the subject of considerable interest. Experimental evidence obtained with cultured lymphoid cell lines in vitro has indicated that 2'-deoxyadenosine, a substrate for ADA, is selectively phosphorylated to dATP in T lymphoblasts'~2 a...