polymorphisms in the β subunit of the high affinity receptor for ige (fcεriβ) and interferon-γ (ifn-γ) genes may influence the risk of asthma. however, the results in asian population are still debatable. we performed a meta-analysis to ascertain the association between the fcεriβ e237g, fcεriβ -109c/t, and ifn-γ 874t/a polymorphisms and asthma in an asian population. databases including pubmed...

background: the affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as elisa. affinity of most igg specific mabs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

â a model is developed for separation of multicomponent gas mixtures in a countercurrent hollow fiber membrane module. while the model’s solution in countercurrent module usually involves in a time consuming iterative procedure, a proper initial guess is proposed for beginning the calculation and a simple procedure is introduced for correcting the guesses, hereby the cpu time is decreased ess...

Abstract Carbon dioxide (CO 2 ) is responsible for approximately 80% of greenhouse gases emission that the main source to global climate change causing notable environmental impacts. Poly (ethylene glycol) diacrylate (PEGDA) have polar PEG repeating units, which provide a strong affinity towards carbon molecules has been blended with 3-aminopropyltrimethoxysilane (APTMS) synthesize membrane CO ...

Procedures for synthesizing a-amino acids on a chip for coordination with transitional metal ions and a His-Tagged protein have been successfully developed as a stable protein microarray. Using the recombinant His-Tagged 3CL-protease (3CL) as a model for attachment to chips containing D-/L-Glu, Asp, Orn, Ser via different transitional metal ions, it was found that the Orn chip was the best of a...

An aptamer can specifically bind to its target molecule, or hybridize with its complementary strand. A target bound aptamer complex has difficulty to hybridize with its complementary strand. It is possible to determine the concentration of target based on affinity separation system for the protein detection. Here, we exploited this property using C-reactive protein (CRP) specific RNA aptamers a...

Adsorption of bioparticles to affinity surfaces involves polyvalent interactions, complicating greatly the recovery of the adsorbed material. A unique system for the efficient binding and release of different cells and particles is described. Affinity-bound bioparticles and synthetic particles are detached from the macroporous hydrogel matrix, a so-called cryogel, when the cryogel undergoes ela...

The efficient separation of butadiene (1,3-C4H6) from C4 hydrocarbons is a critical step in petrochemical processes. However, the traditional cryogenic distillation suffers energy-intensity and serious environmental stress, necessitating development alternative technologies for 1,3-C4H6 separation. Herein, recognition mixed matrix membrane reported via incorporating metal copper encapsulated me...

The interactions of proteins with their immobilized ligands in an electrically charged microenvironment were studied. The binding of lectins to erythrocytes and to affinity matrices was used as a model system. Lectins bind and agglutinate erythrocytes in the presence of at least 10 mM NaCl or 1 mM CaCl2, but not in deionized water. The salt dependence of the agglutination process is due to the ...

Simulated moving bed (SMB) chromatography has been widely studied in past and there have been various operating schemes proposed for efficient separation of binary mixtures. SMB is a promising option of separation because of its capability to achieve efficient separation even when the components differ very little in terms of affinity towards the stationary phase. However, separation of a terna...