Humans are exposed to the hepatotoxic aflatoxin B1 (AFB1) and nephrotoxic ochratoxin A (OTA) through diet. However, kinetic and toxicological data after their co-administration are scarce. In this study, a single oral dose of AFB1 (0.25 mg/kg bw)+OTA (0.5 mg/kgbw) was administered to fasted F344 rats. Blood, liver and kidney were harvested at different timepoints for mycotoxins quantification, ...

The covalent binding of photoactivated aflatoxin B1 ((AFB1) to DNA and its effect on template activity have been investigated. AFB1 in its ground state complexes more preferentially with denatured DNA than with native DNA. The covalent linkage between 3H-AFB1 and DNA under near-ultraviolet light irradiation shows 1 AFB1 per 529 Pi of calf thymus DNA. The photoaddition also induces a conformatio...

BACKGROUND Hepatocellular carcinoma (HCC) is a leading cause of cancer deaths. Aflatoxins, which may play a causative role in 5-28% of HCCs worldwide, are activated in liver cells and induce principally G→T mutations, including the TP53 codon 249(G→T) hotspot mutation. The DNA damage checkpoint response acts as an antitumour mechanism against genotoxic agents, but its role in aflatoxin-induced ...

Background: Aflatoxins are fungal secondary metabolites negatively affecting ruminant performance; however, little information is available on their impact on rumen fermentation. Aims: This study aimed at determining the effects of different concentrations of aflatoxin B1 (AFB1) from Aspergillus flavus on in vitro gas production and ruminal fe...

Aflatoxin B1 (AFB1), the most common mycotoxin in human food and animal feed, produces hepatotoxic, genotoxic and immunosuppressive effects in multiple species. Selenium (Se) has emerged as an important element in the dietary prevention of various toxic agents. The present study was designed to scrutinize the protective effects of sodium selenite on the histological lesions and suppression of m...

Metabolic activation of the hepatocarcinogenic mycotoxin aflatoxin B1 (AFB1) results in the covalent attachment of AFB1 to serum albumin. Digestion of adducted albumin releases AFB1-lysine, a biomarker of exposure status. AFalbumin adducts have been most frequently measured in precipitated serum albumin using an immunoassay (ELISA); however, a sensitive and specific isotope dilution mass spectr...

Aflatoxin B1 (AFB1) is one of the major risk factors for liver cancer globally. A recent study showed that sulforaphane (SF), a potent inducer of phase II enzymes that occurs naturally in widely consumed vegetables, effectively induces hepatic glutathione S-transferases (GSTs) and reduces levels of hepatic AFB1-DNA adducts in AFB1-exposed Sprague Dawley rats. The present study characterized the...

Aflatoxins B1 (AFB1), B2 (AFB2), and G1(AFG1) have been detected in red chili peppers from Chile, Bolivia, and Peru, each of which have a high incidence of gallbladder cancer (GBC) as shown in our previous studies (Tsuchiya el al., 2011; Asai et al., 2012). The association between exposure to AFB1 and the development of liver cancer had already been demonstrated by several studies (e.g., WHO 20...

Background & Aims of the Study: Among aflatoxins, aflatoxin B1 (AFB1) is more toxic and dangerous. The presence AFB1 in poultry feed one most critical health economic problems industry. concern caused by contamination industry transfer toxins from to its products, such as meat, eggs, edible parts. Thus, developing scientific strategies for controlling nutrition protecting public very important....