We have investigated Lf immunoexpression as well as its biological meaning in 71 formalin-fixed, paraffin-embedded surgical samples of endometrial carcinomas (EC); 64 EC were endometrioid type, whereas 7 were non-endometrioid carcinomas. Immunohistochemistry was performed by primary antibodies against Lactoferrin (Lf), estrogen receptor (ER), progesterone receptor (PR) and Ki-67 antigen. Quanti...

The cervix is commonly affected with various conditions including disorders of epithelial maturation, hyperplasia, benign tumors, dysplasia, and malignant neoplasms. Squamous cell carcinomas at all sites, evolve through a sequence of changes occurring in the normal squamous epithelium, ranging from dysplasia of varying degree to frank malignancy. Cell proliferation is an important feature of dy...

AIMS To evaluate whether high numbers of silver staining nucleolar organiser regions (AgNORs) in hepatocytes are associated with increased risk of hepatocellular carcinoma in chronic liver disease. METHODS The quantitative distribution of AgNORs was studied in the liver biopsy specimens of 33 patients with chronic liver disease, 11 of whom developed hepatocellular carcinoma. The interval betw...

Traditional approaches to pathological diagnosis include macroscopic examination and formalin fixation with the preparation of paraffin sections stained with a variety of histochemical techniques. These routine approaches however may be disappointing in the assessment of many conditions and occasionally fail to render a precise diagnosis or provide adequate prognostic information. Consequently ...

AIM: To evaluate the multiple biomarkers of colorectal tumor and their potential usage in early diagnosis of colorectal cancers. METHODS: Multiple biomarkers (DNA contents, AgNOR, PCNA, p53, c-erbB-2) in 10 normal colorectal mucosae, 37 colorectal adenomas and 55 colorectal cancers were analyzed quantitatively in the computed processing imaging system. Discrimination patterns were employed to e...

AIM To evaluate the haemopoietic function of bone marrow blood forming cells in human myelodysplastic syndromes (MDS) by silver staining of nucleolar organiser regions (AgNORs). METHODS Nucleoli were investigated in bone marrow blast cells and in erythroid, granulocytic, and megakaryocytic cells from 12 haematologically healthy subjects, and from 26 patients with MDS, including 14 with refrac...

Aim. The aim of the paper was to evaluate AgNOR parameters for discrimination benign from malignant breast tumors via a new approach - total area/nuclear area (TAA/NA). Material and methods. Three groups, consisting control (n = 14), 18) 28) participants were included in study. staining technique performed both mean number TAA/NA ratio evaluated. Results. While differences between patient group...

The thyroid is an important endocrine gland, which plays a major role in the functions of almost all normal metabolic processes. In present study, by focusing sections at very high magnification (100x, oil immersion level) AgNOR dots are visible as black inside nucleus. Our studies have reported that lesions with less counts more likely to regress compared those and showed its prognostic signif...