Breakdown of HeLa cell DNA begins within 90 min after infection with vaccinia virus at a multiplicity of infection of 2-plaque-forming units per cell, and ends about 7.5 hr after infection. HeLa cell DNA is degraded to a uniform size of 1 to 2 x 10(7) daltons, as judged by alkaline sucrose sedimentation analysis. The rate of host-cell DNA degradation by vaccinia virus increased directly with th...

A partially purified bovine cortical bone acid phosphatase, which shared similar characteristics with a class of acid phosphatase known as tartrate-resistant acid phosphatase, was found to dephosphorylate phosphotyrosine and phosphotyrosyl proteins, with little activity toward other phosphoamino acids or phosphoseryl histones. The pH optimum was about 5.5 with p-nitrophenyl phosphate as substra...

During the course of investigations on riboflavin 5’-phosphate and flavin adenine dinucleotide-hydrolyzing enzymes in green gram (Phaseolus radiatus), we observed that crude extracts possessed high phosphatase activity against a variety of phosphorylated compounds. Despite intensive studies on the animal phosphatases, plant phosphatases have received very little attention. Pfankuch (1) purified...

N-Acetylneuraminic acid is produced by alkaline epimerization of N-acetylglucosamine to N-acetylmannosamine and then subsequent condensation with pyruvate catalyzed by free N-acetylneuraminic acid aldolase. The high-alkaline conditions of this process result in the degradation of reactants and products, while the purification of free enzymes to be used for the synthesis reaction is a costly pro...

Protein A affinity chromatography is a central part of most commercial monoclonal antibody and Fc-fusion protein purification processes. In the last couple years an increasing number of new Protein A technologies have emerged. One of these new Protein A technologies consists of a novel, alkaline-tolerant, Protein A ligand coupled to a macroporous polymethacrylate base matrix that has been optim...

Vibrio sp. V26 isolated from mangrove sediment showed 98 % similarity to 16S rRNA gene of Vibrio cholerae, V. mimicus, V. albensis and uncultured clones of Vibrio. Phenotypically also it resembled both V. cholerae and V. mimicus. Serogrouping, virulence associated gene profiling, hydrophobicity, and adherence pattern clearly pointed towards the non-toxigenic nature of Vibrio sp. V26. Purificati...

The increasing demand for monoclonal antibodies (MAb) as biopharmaceuticals has promoted the development of cell cultures with increased expression levels. As a consequence, the demand for more efficient purification processes has increased. This application note describes a two-step process for MAb purification based on MabSelect SuReTM and CaptoTM adhere, a strong multimodal anion exchanger. ...

Copper mine tailings are the residual products after the purification of precious copper from copper ores, and their storage can create numerous environmental problems. Many researchers have used copper mine tailings for preparation of geopolymer. This paper studies the enhancement of the cementitious activity of copper mine tailings in geopolymer system. First, copper mine tailings are activat...

Most of the companies are not currently manufacturing the detergents alone; they are producing enzyme-based detergents. More than 50% of the detergents produced in the developed countries presently contain enzymes to improve the detergency by removing tough stains. The role of a detergent lipase is to digest the lipidic molecules from the soiled substrates. Most of the chemical detergent ingred...

Highly purified alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] from calf intestine inactivates the glucocorticoid-binding capacity of soluble preparations from mouse fibroblasts (L cells) and rat liver. The unbound receptor is sensitive to inactivation whereas the steroid-bound receptor is unaffected. The ability of the enzyme preparation to ina...