investigated by immunoblot with serum from individuals sensitized to anisakis or shrimp. IgE binding patterns in the immunoblots were used for the identification of major Anisakis allergens, which were analysed by mass spectrometry-based proteomics in ESI-Orbitrap, after separation on SDS-gel. Results: Four new allergen candidates were identified. The first identified allergen was enolase, whic...

Allergic diseases in animals are increasingly gaining importance in veterinary practice and as research models. For intradermal testing and allergen immunotherapy, a good knowledge of relevant allergens for the individual species is of great importance. Currently, the knowledge about relevant veterinary allergens is based on sensitization rates identified by intradermal testing or serum testing...

No data on the relationship between allergic sensitization to animal dander and milk allergens are available in literature in adults. Considering the high rate of allergic sensitization to mammals in atopic adults living in Naples area, we aimed to evaluate if sensitization to milk allergens could be a risk factor for these subjects. From 755 consecutive outpatients examined between May 1 2009 ...

Fungal sensitisation is closely associated with asthma; however, the correlation between fungi and asthma severity remains unclear. The aim of this study was to investigate the severity of asthma in 100 patients with asthma due to fungal and non-fungal allergens. A total of 100 outpatients and inpatients with asthma were selected from 2010 to 2011 and were divided into three groups (mild, moder...

The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study ana...

INTRODUCTION Differentiating between cross-reactivity and double sensitization is still a challenging issue in allergology. AIM To differentiate cross-reactions accompanying latex allergy with the use of the ISAC test. MATERIAL AND METHODS Thirty-nine patients reporting immediate allergic reactions to latex were enrolled into the study (group A). The control group was comprised of 41 patien...

Results Suppression of wheal responses was significant following antihistamine up to 20hr for both HPC and most allergens (15% to 70% wheal size in mm). 1mg/mL histamine appeared negative in some cases under suppression when the 6mg/mL histamine was positive. The lowered wheal size persisted in some subjects up to 72 hrs. Even though the HPC did not appear to be suppressed at some times followi...

Background. In the Republic of Belarus, allergic diseases affect from 10 to 15% population, and there is a steady increase in incidence rhinitis (AR) bronchial asthma (BA). The purpose study was evaluate effect gender age on nature sensitization inhalation allergens residents Grodno region suffering AR, BA combination these (AR + BA). Material methods. Based results skin prick tests with househ...

227 Changing Scenario of Airborne Allergens in Bangalore, India Anand Pendakur, MBBS, DLO and Balaji Ramdas, MBBS. Allergy Asthma ENT Clinic, Indian College of Allergy Asthma and Applied Immunology, Bangalore, India; Community Medicine, Indian Academy of Allergy, Bangalore, India. With phenomenal growth of Bangalore city and a distinct shift of its arboriculture in the last 2 decades, change in...

The conformational stability of B cell epitopes on the 25-kDa group I and 14-kDa group II mite allergens was compared by using heat-treated or chemically denatured allergens to inhibit the binding of native 125I allergens to murine mAb or to human IgE antibodies. Structural changes after treatment were assessed by SDS-PAGE and circular dichroism spectroscopy. Heating for 1 h at greater than 75 ...