To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amp...

the microleakage of cinaally amalgam, manufactured by dr faghihi co. which is a high copper alloy and fine-cut amalgam was evaluated by radioisotope agents. the microleakage of cinaally amalgam was compared with sybraloy amalgam, manufactured by sybron/kerr co. which is a high copper alloy and spherical shape amalgam and is known as a standard amalgam alloy. the results showed that there is no ...

Retroviruses use a cellular tRNA molecule as primer for reverse transcription. The complementarity between the 3' end of this tRNA and a sequence near the 5' end of the viral RNA, the primer-binding site (PBS), allows the primer to anneal onto the viral RNA. During reverse transcription 18 nucleotides of the tRNA primer are copied into the viral cDNA, thereby regenerating the PBS sequence of th...

DNA polymerases amplify DNA fragments through primer extension reactions. However, polymerization behavior of short primers in the primer extension process has not been systematically explored. In this study, we examined the minimal primer length required for primer extension, and the effect of primer length, mismatches and other conditions on DNA polymerization using a non-radioactive method. ...

An example of an object model is given in full in three languages: Alloy, a new notation; Z, a formal specification language; and UML, a modelling notation popular in industry. Basic features of Alloy are explained informally, and briefly justified by comparison of the Alloy version to the UML and Z versions.

This paper presents a compiler from expressive, relational specifications to logic programs. Specifically, the compiler translates the Imperative Alloy specification language to Prolog. Imperative Alloy is a declarative, relational specification language based on first-order logic and extended with imperative constructs; Alloy specifications are traditionally not executable. In spite of this th...

The superplasticity of commercially pure titanium alloy at high temperature was studied and the dynamic recrystallization phenomenon of the alloy was investigated by using electron back scattered diffraction. A two-step deformation method was used to increase the ductility of the alloy from 188% to 243%. 2004 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

The polymerase chain reaction (PCR) is sensitive to mismatches between primer and template, and mismatches can lead to inefficient amplification of targeted regions of DNA template. In PCRs in which a degenerate primer pool is employed, each primer can behave differently. Therefore, inefficiencies due to different primer melting temperatures within a degenerate primer pool, in addition to misma...

The alloy scattering potential is an important parameter in SiGe alloys since it not only affects the velocity-field characteristics for carrier transport, but also allows increased optical transitions by relaxing k-selection rules. In this letter, we report on the velocity-field measurements for relaxed and coherently strained SiGe alloys. The alloy scattering potential is obtained from a care...

We report that the oxygen binding energy of alloy-core@Pt nanoparticles can be linearly tuned by varying the alloy-core composition. Using this tuning mechanism, we are able to predict optimal compositions for different alloy-core@Pt nanoparticles. Subsequent electrochemical measurements of ORR activities of AuPd@Pt dendrimer-encapsulated nanoparticles (DENs) are in a good agreement with the th...