alpha-Protein is a high mobility group protein originally purified from African green monkey cells based on its affinity for the 172-base-pair repeat of monkey alpha-satellite DNA. We have used DNase I footprinting to identify 50 alpha-protein binding sites on simian virus 40 DNA and thereby to determine the DNA binding specificity of this mammalian nuclear protein. alpha-Protein binds with app...

Qing’E pills are a classical formula in traditional Chinese medicine that has the effect of tonifying kidneys and strengthening bones, first appearing tonic formulae prescriptions peaceful benevolent dispensary (10th century CE). To preliminarily elucidate potential mechanisms treatment postmenopausal osteoporosis based on network pharmacology. The principal components corresponding protein tar...

A total of 126 Salmonella spp. isolates from pigs belonging to 13 serotypes (Typhimurium, Derby, Infantis, Enteritidis, Agona, Kaapstad, London, Montevideo, Bredeney, Give, Oritamerin, Schwarzengrund and Tennessee) were tested for sensitivity to 14 antibiotics. Resistance to 1–8 antibiotics was demonstrated in 64 isolates (59.8%), classified into seven serotypes with the most frequent being Sal...

Attempts to measure the platelet-derived growth factor (PDGF) in human plasma resulted in the discovery of a specific plasma binding protein. The 125I-labeled PDGF (125I-PDGF)-plasma binding protein complex retained mitogenic activity but lost reactivity against rabbit anti-PDGF antiserum. Copurification of the plasma binding protein and alpha 2-macroglobulin (alpha 2M) in human plasma, the for...

alpha-Synuclein is an abundant presynaptic protein implicated in neuronal plasticity and neurodegenerative diseases. Although the function of alpha-synuclein is not thoroughly elucidated, we found that alpha-synuclein regulates dopamine synthesis by binding to and inhibiting tyrosine hydroxylase, the rate limiting enzyme in dopamine synthesis. Understanding alpha-synuclein function in dopaminer...

Acute promyelocytic leukemia (APL) is characterized by the translocation, t(15;17) and the expression of a PML/RAR alpha fusion protein that is diagnostic of the disease. There is evidence that PML/RAR alpha protein acts as a dominant negative inhibitor of normal retinoid receptor function and myeloid differentiation. We now show that the PML/RAR alpha fusion product is directly downregulated i...

Molecular cloning of cDNAs encoding alpha-subunits of guanine nucleotide-binding regulatory proteins (G-proteins) has revealed the existence of nine species of alpha-subunits. We have identified two additional G-protein alpha-subunits, which we refer to as GL1 alpha and GL2 alpha, by isolating bovine liver cDNA clones that cross-hybridized at reduced stringency with bovine Gi1 alpha-subunit cDN...

Nowadays, air pollution is one of the major problems in developed and developing countries. In recent years, effects of air pollution on neuroinflammatory diseases such as Alzheimer disease and Parkinson disease have been studied. Researches on polluted cities citizens indicate increasing in central nervous system (CNS) inflammatory factors in comparison with clean cities; also air pollution ex...