نتایج جستجو برای: amanitin
تعداد نتایج: 523 فیلتر نتایج به سال:
Influenza virus polypeptides were not synthesized in wild-type CHO-S-infected cells in the presence of alpha-amanitin, but were synthesized in CHO-Amal cells, a mutant cell line whose DNA-dependent RNA polymerase II is specifically resistant to this drug, indicating that this cellular enzyme is involved in influenza virus replication. The results of experiments designed to detect viral polypept...
A procedure is described for the purification of the alpha-amanitin-sensitive DNA-dependent RNA polymerase [EC 2.7.7.6] from wheat germ. Solubilization of the enzyme activity was achieved by sonication of a crude extract in a high-salt buffer. Purification involved precipitation with protamine sulphate and (NH(4))(2)SO(4), chromatography on DEAE-cellulose and phosphocellulose, and sucrose gradi...
Evidence has been presented that a amanitin, the potent bicyclic polypeptide toxin of the toad stool amanita phalloides inhibits transcription by purified RNA polymerase from rat liver nuclei. This inhibition is highly specific for the mammalian enzyme, since it does not impede the same reaction catalyzed by the enzyme from E. coli thus rendering an interaction between the template and inhibit...
BACKGROUND Although some Japanese Galerina species poisonings manifest as gastrointestinal symptoms followed by late-onset hepatorenal failure (phalloides syndrome), the toxin responsible for this has not been determined. CASE REPORT We report a 6-year-old boy who developed characteristic cholera-like diarrhea and late-onset severe hepatic deterioration after eating mushrooms, later identifie...
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