In the presence of the soluble polypeptide antigens ovalbumin and keyhole limpet hemocyanin, purified mouse spleen dendritic cells induce the secretion of IL-2 by antigen-specific T cell hybridomas. This response is H-2 restricted and can be specifically inhibited by monoclonal anti-I-A. These data indicate that dendritic cells can present soluble antigen to H-2-compatible T cells.

In a previous paper (1) we described the appearance of a soluble circulating antibody-antigen complex in rabbits after single intravenous injections of large doses of bovine plasma albumin or bovine plasma gamma globulin, or after intramuscular injections of these agents with Freund's adjuvants. The soluble complex was demonstrated in the serum by brief exposure to alkali in the cold followed b...

Active infection by T. gondii was evaluated by immunoassay for soluble SAG-1 (p30), the major surface antigen from T. gondii, specific antibodies and immune complexes in human cerebrospinal fluid (CSF) samples. A total of 263 samples of CSF were collected from hospitalized patients presenting neurological disorders and analyzed for antibodies to HIV. Patients were divided into two groups: HIV p...

Separate antisera were raised against keratocyst, dentigerous cyst, and radicular cyst fluids and used to analyse a range of fluids from cysts of known type. Samples were subjected to crossed immunoelectrophoresis into homologous antiserum through an intermediate gel containing antibody to whole human serum to screen out serum derived components. A major antigen, designated X, which seems to be...

Induction of inducible nitric oxide synthase in mononuclear phagocytes by IFN-γ and innate tumor necrosis factor (TNF) provide the basis for an effective immune response to the intracellular parasite Leishmania (L.) major. In previous experiments, we observed a fatal visceral form of leishmaniasis in L. major-infected C57BL/6 TNF(-/-) mice. To further delineate the protective function of TNF an...

BACKGROUND Currently there is no vaccine available in use against any form of leishmaniases worldwide. OBJECTIVE To assess potential of a live-attenuated Leishmania major promastigates, for protection against a challenge infection with L. major in BALB/c mice. DESIGN A laboratory based study. SETTING Study was carried out at Centre for Biotechnology Research and Development, Kenya Medical...

Recent evidence revealed that presentation of soluble antigens is governed by the endocytosis mechanisms that determine the intracellular routing of the endocytosed antigens. Soluble antigens intended for classical exogenous MHC-II-restricted presentation are internalized into lysosomes. Soluble antigens destined for crosspresentation are taken up by distinct endocytosis mechanisms and are conv...

background: cationic immune stimulating complexes (pluscoms) are particulate antigen delivery systems. pluscoms consist of cationic immunostimulatory complexes (iscoms) derivatives and are able to elicit in vivo t cell responses against an antigen. objective: to evaluate the effects of pluscoms containing leishmaniamajor antigens (sla) on the type of immune response generated in the murine mode...