Auxotrophic mutants of the actinomycete Amycolatopsis methanolica requiring l-Phe or l-Tyr were isolated and identified as strains lacking prephenate dehydratase (strain GH71) or arogenate dehydrogenase (strain GH70), respectively. A. methanolica thus employs single pathways only for the biosynthesis of these aromatic amino acids. Anion-exchange chromatography of extracts revealed two peaks wit...

In this study, we investigate the potential of Moricandia arvensis methanol leaf extract (MeOHL) on calpain activity, melanin biosynthesis and DNA mutagenicity. Cytotoxic effect and measurement of reactive oxygen species (ROS) induced by lucigenin in colorectal cells (BE) were also determined. In addition the chemical analysis of the extract was also studied and the chemical profile illustrates...

BACKGROUND AND PURPOSE Microbial tannase (tannin acyl hydrolase, EC 3.1.1.20), a hydrolysable tannin-degrading enzyme, has gained importance in various industrial processes, and is used extensively in the manufacture of instant tea, beer, wine, and gallic acid. Tannase is an inducible enzyme, and hydrolysable tannin, especially tannic acid, is the sole inducer. This study is of the effect of va...

Glucose may be metabolized through the Embden-Myerhof glycolytic sequence to the threecarbon compounds, pyruvate and lactate. Alternatively, some of the glucose may be converted (a) to pentose via the hexose monophosphate shunt (1~) or (b) to serine and glycine by a series of reactions which probably branch from glycolysis at the level of phosphoglyceric acid (1, ~, 7, 8). The pyruvate and lact...

Glucose may be metabolized through the Embden-Myerhof glycolytic sequence to the threecarbon compounds, pyruvate and lactate. Alternatively, some of the glucose may be converted (a) to pentose via the hexose monophosphate shunt (1~) or (b) to serine and glycine by a series of reactions which probably branch from glycolysis at the level of phosphoglyceric acid (1, ~, 7, 8). The pyruvate and lact...

The antibiotic cycloheximide inhibits chain initiation and the extension of nascent protein chains (6). One might therefore expect cycloheximide to have a number of indirect effects depending on the duration of the treatment and the concentrations used. For example, in several in vivo systems cycloheximide has been shown to block protein synthesis completely at concentrations of 5 to 10 ,ug/ml ...

Glucose may be metabolized through the Embden-Myerhof glycolytic sequence to the threecarbon compounds, pyruvate and lactate. Alternatively, some of the glucose may be converted (a) to pentose via the hexose monophosphate shunt (1~) or (b) to serine and glycine by a series of reactions which probably branch from glycolysis at the level of phosphoglyceric acid (1, ~, 7, 8). The pyruvate and lact...

Glucose may be metabolized through the Embden-Myerhof glycolytic sequence to the threecarbon compounds, pyruvate and lactate. Alternatively, some of the glucose may be converted (a) to pentose via the hexose monophosphate shunt (1~) or (b) to serine and glycine by a series of reactions which probably branch from glycolysis at the level of phosphoglyceric acid (1, ~, 7, 8). The pyruvate and lact...

The importance of glycine in animal and plant metabolism as a precursor of many nitrogenous compounds, such as creatine, porphyrins, chlorophyll, uric acid and nucleic acid purines is now well established (for a recent review, see Arnstein, 1954). Relatively little is known, however, about the metabolic origin of this amino acid from non-nitrogenous precursors. That glycine can be synthesized b...

Gamma-aminobutyric acid (GABA) is a non-protein amino that widely present in organisms. In particular, lactic bacteria (LAB) stand out this regard. The biosynthesis of GABA microorganisms mainly regulated by pH, which usually has the most pronounced effect on fermentation process. Also, production, affects media composition, time, and temperature. important positive functions human health. it k...