Studies in vitro suggested that inflammatory cytokines could cause myocardial dysfunction. However, the detailed mechanism for the cytokine-induced myocardial dysfunction in vivo remains to be examined. We thus examined this point in our new canine model in vivo, in which microspheres with and without IL-1beta were injected into the left main coronary artery. Left ventricular ejection fraction ...

Studies on the Reduction of Aromatic Nitro Groups in Human and Rodent Placental Homogenates. MONT R. JUCHAU 1 Metabolism of Some Phenylethylamines and Their fl-Hydroxylated Analogs in Brain. GEORGE R. BREESE, THOMAS N. CHASE AND IRWIN J. KOPIN 9 A Specific and Sensitive Assay for Aminoguanidine: Its Application to a Study of the Disposition of Aminoguanidine in Animal Tissues. M. A. BEAVEN, J. ...

The purpose of these experiments was to investigate a potential role for vascular endothelial growth factor (VEGF) in mediating vascular dysfunction induced by increased glucose flux via the sorbitol pathway. Skin chambers were mounted on the backs of Sprague-Dawley rats and 1 wk later, granulation tissue in the chamber was exposed twice daily for 7 d to 5 mM glucose, 30 mM glucose, or 1 mM sor...

This study was designed to determine the role of endogenous prostaglandins (PG) and nitric oxide (NO) in the lipopolysaccharide (LPS)-induced ACTH and corticosterone secretion in conscious rats. LPS (0.5 and 1 mg/kg) given i.p. stimulated the hypothalamic-pituitary-adrenocortical (HPA) activity measured 2 h later. A non-selective cyclooxygenase inhibitor indomethacin (10 mg/kg i.p.), piroxicam ...

1. Histamine catabolism in vivo was studied in mice subjected to various forms of pretreatment; tissues from mice killed 2.5 min after intravenous injection of (14)C-histamine were assayed for (14)C-histamine, (14)C-methylhistamine and total (14)C.2. Pretreatment of mice with aminoguanidine, an inhibitor of diamine oxidase, strongly increased levels of (14)C-histamine in intestine; pretreatment...

Certain liver metabolic diseases point to the presence of disturbances in glycogen deposition. Epinephrine raises the cAMP level that activates protein kinase A leading to the activation of phosphorylase and glycogen breakdown. In the present report, we sought to investigate whether NO is produced during adrenoceptor agonist-induced glycogenolysis in rat hepatocytes in cultures. Isolated glycog...

P-glycoprotein (P-gp), one of the important drug-efflux pumps, is known to affect pharmacokinetics and pharmacodynamics of P-gp substrate drugs. We have previously reported that intestinal P-gp expression levels are transiently decreased in streptozotocin (STZ)-induced type 1 diabetic mouse model. Herein, we examined the analgesic effects of orally administered morphine and its pharmacokinetic ...