A soluble thiosulphate-oxidizing multi-enzyme system, precipitated from a crude cell extract of Thiobacillus A2 with ammonium sulphate, has been resolved into four essential components by DEAE-Sepharose chromatography, gel filtration of Sephadex G-1 00 and G-200, hydrophobic interaction chromatography on phenyl-Sepharose and preparative isoelectric focusing. Oxidation of thiosulphate to sulphat...

Four simple and sensitive methods for the assay of prazosin hydrochloride (PRH) are developed. These methods are based on the formation of coloured species by treating it either with excess N-bromosuccinimide (NBS) and determining the unconsumed NBS with p-N-methyl aminophenol sulphate (metol)-sulphanilamide (SA) reagent (method A, lambda(max) 520 nm): with 3-methyl-2-benzothiazolinone hydrazon...

Green mature fruits of mango cv. SS-1 (Kala Chaunsa) were immersed for 10 minutes in 2.5, 5.0 or 7.5% calcium chloride (CaCl2.2H2O), calcium sulphate (CaSO4.2H2O) or calcium ammonium nitrate {Ca(NH4NO3)2} solutions. A control was also included in which fruits were dipped in fresh water for 10 minutes. The fruits were ripened at ambient temperature (25 ± 3 C) in boxes lined and covered with news...

Comparison of the production of pectin methylesterase (PME) by Aspergillus niger from apple pomace in solid-state (SSF) and submerged fermentation (SmF) was made for higher PME production. pH value of 4.0, incubation temperature of 25 °C and incubation period of 96 h were found optimum in both SSF and SmF, while optimum dilution levels were 1:3 and 1:6, respectively. Ammonium sulphate in SSF an...

This study compares the chemical composition of rainwater samples collected at two sampling sites, the first situated in the Rijeka city centre and the second in a suburban site 120 m above the sea level. The rainwater samples were analysed for precipitation weighted average concentrations of hydrogen, sulphate, nitrate, chloride, ammonium, sodium, potassium, calcium, and magnesium. The results...

The presence of cytosolic S-adenosylmethionine-dependent N-methyltransferase(s) activity(ies) capable of converting phosphoethanolamine into phosphocholine has been recently demonstrated in the rat brain. At least two enzymes are involved in the methylation of phosphoethanolamine to phosphocholine and these are separable by ammonium sulphate fractionation. The enzyme catalysing the last step of...

The soluble fraction of homogenates of synchronous CMorg#a /w^ca was tested for carbohydrate-lyzing activities. With isolated cell walls and /?-l,4-mannan or carboxymethyl cellulose as substrates, a sharp increase in activity occurred shortly before release of the daughter cells followed by a decline during release. The lytic activities were partially purified by ammonium sulphate precipitation...

Application of cavity ring down (CRD) spectrometry for measuring the optical properties of pure and mixed laboratory-generated aerosols is presented. The extinction coefficient (α ext), extinction cross section (σ ext) and extinction efficiency (Q ext) were measured for polystyrene spheres (PSS), ammonium sulphate ((NH 4) 2 (SO 4)), sodium 5 chloride (NaCl), glutaric acid (GA), and Rhodamine-59...