نتایج جستجو برای: amplification refractory mutation system

تعداد نتایج: 2569291  

2015
A Ghotaslou F Nadali B Chahardouli N Alizad Ghandforosh SH Rostami K Alimoghaddam A Ghavamzadeh

BACKGROUND Myeloproliferative disorders are a group of diseases characterized by increased proliferation of myeloid lineage. In addition to JAK2V617F mutation, several mutations in the c-MPL gene have been reported in patients with philadelphia-negative chronic myeloproliferative disorders that could be important in the pathogenesis of diseases. The aim of the present study was to investigate t...

Journal: :Acta biochimica Polonica 2013
Marcin M Machnicki Eliza Glodkowska-Mrowka Tomasz Lewandowski Rafał Ploski Pawel Wlodarski Tomasz Stoklosa

BRAF mutation testing is one of the best examples how modern genetic testing may help to effectively use targeted therapies in cancer patients. Since many different genetic techniques are employed to assess BRAF mutation status with no available comparison of their sensitivity and usefulness for different types of samples, we decided to evaluate our own PCR-based assay employing the amplificati...

2014
Zhijie Wang Rui Chen Shuhang Wang Jia Zhong Meina Wu Jun Zhao Jianchun Duan Minglei Zhuo Tongtong An Yuyan Wang Hua Bai Jie Wang

BACKGROUND Among advanced non-small cell lung cancer (NSCLC) patients with an acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKI), about 50% carry the T790M mutation, but this frequency in EGFR-TKI-naïve patients and dynamic change during therapy remains unclear. This study investigated the quantification and dynamic change of T790M mutation in plasma c...

Journal: :International journal of clinical and experimental pathology 2015
Huanli Duan Junliang Lu Tao Lu Jie Gao Jing Zhang Yan Xu Mengzhao Wang Huanwen Wu Zhiyong Liang Tonghua Liu

BACKGROUND The aims were to compare the consistency of epidermal growth factor receptor (EGFR) mutations in the plasma and tumor tissue of NSCLC patients, and to explore the prognostic significance of plasma EGFR mutation status in tyrosine kinase inhibitors (TKIs)-treated patients with tumor EGFR mutation. METHODS We evaluated EGFR gene (exons 18, 19, 20 and 21) mutation status in paired pla...

2014
J Manoochehri R Masoumi Dehshiri H Faraji S Mohammadi H Dastsooz T Moradi E Rezaei Kh Sadeghi M Fardaei

BACKGROUND Wilson disease (WD) is a rare autosomal recessive disorder, which leads to copper metabolism, due to mutations in ATP7B gene. The gene responsible for WD consists of 21 exons that span a genomic region of about 80 kb and encodes a copper transporting P-type ATPase (ATP7B), a protein consisting of 1465 amino acids. Identifying mutation in ATP7B gene is important to find carrier indivi...

Journal: :Agronomy 2022

Amylose content (AC) is the major indicator of rice eating and cooking quality (ECQ). Its synthesis in endosperm mainly regulated by protein, granule-bound starch synthase 1, which encoded waxy gene (Os06g0133000, LOC_Os06g04200). The diversity AC largely attributable to allelic variation at Wx locus development effective accurate functional molecular markers target variant alleles crucial bree...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2016
Wendy Chang Andrew S Brohl Rajesh Patidar Sivasish Sindiri Jack F Shern Jun S Wei Young K Song Marielle E Yohe Berkley Gryder Shile Zhang Kathleen A Calzone Nityashree Shivaprasad Xinyu Wen Thomas C Badgett Markku Miettinen Kip R Hartman James C League-Pascual Toby N Trahair Brigitte C Widemann Melinda S Merchant Rosandra N Kaplan Jimmy C Lin Javed Khan

PURPOSE We undertook a multidimensional clinical genomics study of children and adolescent young adults with relapsed and refractory cancers to determine the feasibility of genome-guided precision therapy. EXPERIMENTAL DESIGN Patients with non-central nervous system solid tumors underwent a combination of whole exome sequencing (WES), whole transcriptome sequencing (WTS), and high-density sin...

Background The X-linked cyclin-dependent kinase like 5 (CDKL5/STK9) gene has been shown to be responsible for a severe encephalopathy condition characterized by early onset of epilepsy and severe developmental delay. CDKL5 mutations have been shown to be more frequent among female patients. Results Here we report a 6- month male patient, second child of a healthy non consanguineous in the Irani...

2014
Zizhen Ming Dongxian Jiang Qin Hu Xiaojing Li Jie Huang Yifan Xu Yalan Liu Chen Xu Xiuguo Hua Yingyong Hou

BACKGROUND The PIK3CA gene mutation was found to associate with prognosis and might affect molecular targeted therapy in esophageal carcinoma (EC). The aim of this study is to compare different methods for analyzing the PIK3CA gene mutation in EC. METHODS Genomic DNA was extracted from 106 surgically resected EC patient tissues. The PIK3CA mutation status (exons 9 and 20) were screened by mut...

2016
Hongxuan Zhou Yun Dai Liqun Zhu Chun Wang Xiaodong Fei Qin Pan Juxiang Chen Xianqing Shi Yanfeng Yang Xiaoxing Tao Pinghuai Shi

OBJECTIVE To evaluate treatment response, survival, and the associations between KRAS mutation status and tumour expression levels of BRCA1, TYMS and SRC retrospectively in a cohort of patients with non-small cell lung cancer (NSCLC), treated exclusively with conjunctive platinum-based doublet chemotherapy. METHODS KRAS mutation status was determined via amplification refractory mutation and ...

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