The Southern Ocean has absorbed most of the excess heat associated with anthropogenic greenhouse gas emissions. Since observations are sparse in certain regions and seasons, much our knowledge ocean uptake is based on climate model simulations. However, models still inadequately represent some properties clouds, they have not identified mechanisms by which clouds may affect (SOHU). Here, we use...

Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan(®) technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This method is widely used to identify global differences between the expression of 100s of miRNAs acros...

Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030 Ever since it was shown that PCR could simultaneously amplify multiple loci in the human dystrophin gene, (1) multiplex PCR has been firmly established as a general technique. A short list of multiplex PCR applications now includes pathogen identification, gender screening, linkage analysis, forensic studies, tem...

Time Division Multiple Access (TDMA) amplify and forward based protocols have been investigated previously by various researchers. However, the gap is still there to investigate the performance of these protocols using inter-relay communication in order to improve the diversity order at destination over Rician fading channel. Therefore, the aim of this paper is to propose TDMA based amplify and...

Long-range PCR remains a flexible, fast, efficient and cost-effective choice for sequencing candidate genomic regions in a small number of samples, especially when combined with next-generation sequencing (NGS) platforms. Several long-range DNA polymerases are advertised as being able to amplify up to 15 kb or longer genomic DNA. However, their real-world performance characteristics and their s...

To study functional diversity of proteins encoded from a single gene, it is important to distinguish the expression levels among the alternatively spliced variants. A variant-specific primer pair is required to amplify each alternatively spliced variant individually. For this purpose, we developed a new feature, homolog-specific primer design (HSPD), in our high-throughput primer and probe desi...