Kernels of Klages barley (Hordeum vulgare L.) were germinated for 1 to 4 days on moist sand at 18 degrees C. Representative kernels from each time period were dissected to give the following fractions: scutellum, subscutellar endosperm, aleurone-scutellum interface, remaining aleurone, subaleurone endosperm, and core endosperm. These tissues were analyzed for alpha-amylase components by isoelec...

We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the n...

Considering the weak gel of tapioca starch and generally incomplete gel-advancing mechanism α-amylase modification, two commercial α-amylases (named A- N-amylase) with different reaction patterns were used to improve strength cross-linked starch. SEM showed that A-amylase exhibited surface erosion pattern, compared an extensive pattern N-amylase. XRD, SAXS, HPGPC-MALLS-RI revealed mainly attack...

An elderly lady with amylase-rich ascites is presented, whose isoenzyme estimation revealed salivary type amylase. Tumour hyperamylasemia is an important group among the nonpancreatic causes of elevated amylase.

Amylase transcription in the human salivary gland results from the evolutionary juxtaposition of two inserted elements, a gamma-actin pseudogene and an endogenous retrovirus, to create an unusual salivary-specific promoter. We utilized these structures as molecular tags to characterize the amylase genes in extant primates by polymerase chain reaction amplification of promoter fragments from gen...

BACKGROUND & AIM Alpha-amylase in both blood and saliva has been used as a diagnostic parameter. While studies examining alpha-amylase activity in saliva have shown that it is sensitive to physiological and psychological challenge of the adrenergic system, no challenge studies have attempted to elucidate the role of the adrenergic system in alpha-amylase activity in blood. We set out to examine...

Pancreatic and salivary amylase/creatinine clearance ratios in patients with various degrees of renal impairment were compared with those obtained for control subjects. In chronic renal insufficiency (mean GFR 30 ml/min +/- 15 SD; n = 13) the clearance ratios for pancreatic (mean 3.5 +/- 1.85 SD) and salivary (mean 2.3 +/- 1.3 SD) amylase were significantly higher (P less than 0.05) than those ...

The flux of alpha-amylase (1,4-alpha-D-glucan glucanohydrolase; EC 3.2.1.1) across the basolateral membrane of the acinar cell was measured in the cell-to-bath direction using the whole rabbit pancreas in organ culture. This in vitro preparation is polarized so that apical and basolateral secretions can be collected separately. The unstimulated amylase flux from cell to bath was substantial at ...

The cloning of a-amylase gene of S . occidentalis and the construction of starch digestible strain of yeast, S . cerevisiae AS. 2.1364 with ethanol-tolerance and without auxotrophic markers used in fermentation industry were studied. The yeast/E . coli shuttle plasmid YCEpl partial library of S. occidentalis DNA was constructed and a-amylase gene was screened in S. cerevisiae by amylolytic acti...

Th e paper presents numerical comparison of two methods of estimation of nonallelic interaction of QTL eff ects. In the fi rst method we assume that we observe only the plant phenotype, while in the second method we have additional information from the molecular markers observations. In this paper we analysed phenotypic data on 120 barley doubled haploid lines, derived from cross Clipper × Saha...