Anaerobic fungi produce extracellular hydrolytic enzymes that facilitate degradation of cellulose and hemicellulose in ruminants. The purpose this work was to study the impact three different anaerobic fungal species (Anaeromyces mucronatus YE505, Neocallimastix frontalis 27, Piromyces rhizinflatus YM600) on hydrolysis two lignocellulosic substrates, corn (Zea mays L.) silage reed (Phragmites a...

The present work aimed to investigate the microbial dynamics during the anaerobic treatment of the azo dye blue HRFL in bench scale upflow anaerobic sludge bed (UASB) reactor operated at ambient temperature. Sludge samples were collected under distinct operational phases, when the reactor were stable (low variation of color removal), to assess the effect of glucose and yeast extract as source o...

Mathematical modelling of anaerobic digestion process has been used to give new insights regarding dynamics of the long chain fatty acids (LCFA) inhibition. Previously published experimental data, including batch tests with clay mineral bentonite additions, were used for parameter identification. New kinetics were considered to describe the bio-physics of the inhibitory process, including: i) a...

Bio-based organic acids are an eco-friendly alternative to petroleumderived products. In this work, the production of organic chemicals was investigated for the first time in the Cosenzaea myxofaciens species using hydrolysed lignocellulosic biomass from Arundo donax. The strain C. myxofaciens BPM1, isolated from bovine rumen, was able to produce a high amount of lactic acid, followed by acetic...

Fusarium oxysporum is one of the few filamentous fungi capable of fermenting ethanol directly from plant cell wall biomass. It has the enzymatic toolbox necessary to break down biomass to its monosaccharides and, under anaerobic and microaerobic conditions, ferments them to ethanol. Although these traits could enable its use in consolidated processes and thus bypass some of the bottlenecks enco...

Thermo-chemical pre-treatment improves the solubilization of the lignocellulosic biomass. Therefore, it could improve biogas production from press mud, the solid residue from sugar cane juice filtration which contains up to 85 % insoluble organic matter. The impact of thermo-alkaline pretreatment conditions (100 o C, Ca(OH)2) on press mud was investigated for different times and concentrations,...

The influence of dilution rate on the production of biomass, ethanol, and invertase in an aerobic culture of Succharornyces carlsbergensis was studied in a glucoselimited chemostat culture. A kinetic model was developed to analyze the biphasic growth of yeast on both the glucose remaining and the ethanol produced in the culture. The model assumes a double effect where glucose regulates the flux...

Different mixtures were digested in a single-stage, batch, mixed, laboratory scale mesophilic anaerobic digester at the Biomass Research Centre Laboratory (University of Perugia). The yield and the composition of biogas from the different substrates were evaluated and the cumulative curves were estimated. Two experimental campaigns were carried out, the first on three mixtures (chicken, pig and...

Microalgal biomass grown in wastewater treatment raceway ponds may be valorised producing bioenergy through anaerobic digestion. However, pretreatment techniques seem to be necessary for enhancing microalgae methane yield. In this study, hydrothermal pretreatment was studied prior to batch and continuous reactors. The pretreatment increased organic matter solubilisation (8-13%), anaerobic diges...

Biomass energy represents a huge supplement for meeting current energy demands. A hyperspectral imaging system covering the spectral range of 874-1734 nm was used to determine the pH value of anaerobic digestion liquid produced by water hyacinth and rice straw mixtures used for methane production. Wavelet transform (WT) was used to reduce noises of the spectral data. Successive projections algo...