A simple and efficient method has been developed for the synthesis of alpha-aminonitriles by a one-pot three- component condensation of aldehydes, amines, and potassium cyanide in acetonitrile in the presence of a catalytic amount of CoCl2 at room temperature.

Experiments were designed to investigate the metabolism of Cyclophosphamide in vitro and in vivo following the administration of known stimulators and depressors of rat hepatic microsomal mixed-function oxidase activity, the antitumor efficacy of Cyclophosphamide as a function of its metabolism, and the toxicity of Cyclophosphamide as a function of its metabolism. Regarding the metabolism of Cy...

Experiments were designed to investigate the metabolism of Cyclophosphamide in vitro and in vivo following the administration of known stimulators and depressors of rat hepatic microsomal mixed-function oxidase activity, the antitumor efficacy of Cyclophosphamide as a function of its metabolism, and the toxicity of Cyclophosphamide as a function of its metabolism. Regarding the metabolism of Cy...

Low O(2) conditions were obtained by flowing N(2) through the solution in which the tomato plants (Lycopersicon esculentum Mill cv Heinz 1350) were growing. Time course experiments revealed that low O(2) treatments stimulated 1-aminocyclopropane-1-carboxylate (ACC) synthase production in the roots and leaves. After the initiation of low O(2) conditions, ACC synthase activity and ACC content in ...

Induction of coumarin 7-hydroxylation, catalyzed by CYP2A5 in mice and CYP2A6 in humans by various known in vivo murine inducers and modifiers, was compared in human and mouse hepatocytes in culture. Phenobarbital and rifampicin were efficient inducers (up to 10-fold induction) after 48-h treatment in murine cultured hepatocytes, whereas the enzyme activity in human hepatocytes was much more re...

In recent years increasing awareness of the importance of water in our national economy has focused attention on the significance of forests in this important phase of conservation of our natural resources. Much has been written in the past 30 years concerning various aspects of the water relations of woody plants, yet available data concerning total water loss from trees and forests are inadeq...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Human CD133 cell isolation 3.2. Cell culture 3.3. Acetylated LDL uptake and UEA-1 lectin binding site labeling 3.4. Tubule formation 3.5. Flow cytometric analysis of cell surface markers 3.6. Migration assay 3.7. Cell wound healing 3.8. Cell proliferation Assay 3.9. Measurement of VEGF, FGF, and HIF-1α protein by ELISA 3.10. Real time PC...

1,1-diacetates(acylals) were prepared by direct condensation of various aldehydes with acetic anhydride using dipyridine cobalt chloride (copy2cl2) as an efficient and green catalyst under solvent-free conditions at room temperature. the important features of this catalyst method are that the catalyst is solid, stable at high temperatures, soluble in water, stable in air, immiscible in common o...

E2F6, a potent transcriptional repressor, plays important roles in cell cycle regulation. However, roles of E2F6 in hypoxia-induced apoptosis are unknown. Here, we demonstrated biological functions of E2F6 in hypoxia-induced apoptosis and regulatory pathways. During hypoxia (CoCl(2), 800 microM)-induced human embryonic kidney 293 cell apoptosis, E2F6 expression was down-regulated with concurren...