The objective of this study was to increase understanding about the mechanism by which polyamines (PAs) promote the conversion of embryogenic calli (EC) into somatic embryos in cotton (Gossypium hirsutum L.). We measured the levels of endogenous PAs and H2O2, quantified the expression levels of genes involved in the PAs pathway at various stages of cotton somatic embryogenesis (SE), and investi...

Somatic embryogenesis was achieved in four leguminous tree species, that is, Acacia catechu, Acacia arabica, Hardwickia binata, and Dalbergia sissoo using immature zygotic embryos as explants on Murashige and Skoog (MS) medium supplemented with 0.25-1.0 mg/l Kn (kinetin) and 2.0-3.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid) or NAA (1-napthaleneacetic acid) and 3% sucrose. MS medium containing...

In this study, cell-suspension culture of strawberry (Fragaria × ananassa), cultivars Camarosa, and Sweet Charlie has been established. Embryogenic callus was induced by incubating the in vitro juvenile leaf explants on medium, containing 2-mg/l picloram at dark. Suspension culture was initiated from 4-week-old embryogenic calli in the liquid MS medium with 1-mg/l 2,4-D and 2-mg/l picloram. Sus...

The peppers or ajies (Capsicum spp.) are one of Peru's main productive crops, which is why it among the top ten countries in production and exports. Among species cultivated Peru Capsicum pubescens Ruiz & Pav., commonly called rocoto, represents most critical local national gastronomy. rocoto ecotype "Selva" "de Monte" characterized by its large size used to prepare relleno arequipeño. This...

The conventional methods of propagation of bamboos sexual as well as vegetative have lots of problems that restrict their multiplication in large numbers. Bamboo is now a commercial crop and hence there is shortage of availability of planting stock material. To overcome this tissue culture offers rapid and reliable method to produce plants in large number. Tissue culture protocol has been devel...

Evapotranspiration, which includes the evaporation of water from soils, may rise as a result climate change and rising temperatures. The outcome is that evaporates but salt stays in soil, raising its saltiness. growth development oil palm are also affected by stress, decades time frame required with conventional breeding due to reproductive biological reasons long-time life span. However, facil...

Pruacan is a rare medicinal plant endemic to the Dieng Plateau of Central Java, Indonesia which has properties as an aphrodisiac. Increasing genetic variability ofthis in order support breeding programs and increase productivity active ingredients this important. This study aimed evaluate effect chemical mutagen EMS on growth development vitro culture pruacan lethal dose determination. Embryoge...

Abstract The Aceh patchouli plant ( Pogostemon cablin . Benth) is a plantation that produces essential oils with great potential, known as oil. use of in vitro culture techniques on plants needs to be developed meet the seeds. This study aimed analyze effect growth regulators (PGRs) cytokinins 6-Benzyl Amino Purine (BAP) and Thidiazuron (TDZ) auxin 1-Naphthaleneacetic Acid (NAA) organogenesis a...

Over the past decades, plant cryopreservation technologies have been evolving rapidly, opening the door to the possibility of long-term storage of valuable genetic resources of many crop and forest species. From the original slow-cooling approach, research has moved to easier and more reproducible techniques which allow a complete vitrification of extraand intra-cellular liquids through the dir...