In this work, we fabricated a novel photoelectrochemical immunosensor for assay of DNA methylation, where Bi2S3 nanorods were used as photoelectric conversion material, MBD1 protein (a kind of methyl bonding domain protein) was used as DNA methylation recognizing unit, anti-his tag antibody was used to further inhibit the photocurrent and increase the detection sensitivity. The results demonstr...

background: mycobacterium tuberculosis is a major cause of mortality and morbidity worldwide. infection with this bacterium is known to induce the development of autoantibodies of which a few are also known to be diagnostic markers for some other diseases. antineutrophil cytoplasmic antibodies (anca's) are among those autoantibodies used in clinical setting for diagnosing systemic vasculit...

Graphical Abstract The cover pictures shows examples of bioorthogonal chemistry and glycoscience integration made possible through methods pioneered by Carolyn Bertozzi. Metabolic oligosaccharide engineering enables the detection glycan structures in living systems, while facile construction glycoconjugates allows for installation defined glycoproteins cutting-edge antibody tools.

Using a high-affinity antibody for estradiol, thermodynamic and experimental limitations on detection limits for competitive capillary electrophoretic immunoassays were examined. Theoretical modeling of the dose-response curves for such assays allowed for optimization of experimental conditions. Through the examination of experimental and theoretical results generalizations could be made as to ...

The Histoplasma antigen immunoassay utilizes an antibody sandwich method that provides a rapid and reliable means of diagnosing the more severe forms of histoplasmosis. Inhibition assays have been developed for antigen detection and offer at least one potential advantage, namely, reduced antibody requirements. We have developed an inhibition assay using the polyclonal antibody employed in our s...

BACKGROUND The p16INK4a is a protein that expressed in Liquid-based cervical cytology specimens and has been proved link to cervical cancer. The p16INK4a could be detection by piezoelectric immunosensor and the immobilization of the p16INK4a antibody influence the sensitivity of the piezoelectric immunosensor. MATERIALS AND METHODS 5μL mouse polyclonal antibody against p16INK4a was bound onto...

antibodies are a diverse class of glycoproteins that specially bind with antigen and elicit a number of secondary responses in vivo. most of the applications of antibodies in basic research diagnosis and immunotherapy rely on labeling of the antibody with radiopharmaceuticals. radiolabeled antibodies are a new class of imaging agents for the detection of sites of disease. true biodistribution o...

definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. to evaluate the efficacy of dot-elisa for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. the specimens were also examined after centrifugation by ...

in this study one lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in pbs, sonication and phenol extraction. tumor antigen detection relies on immunoprecipitation followed by sds-page. in this experiment streptococcus pyogenes was used for purification of immune complexes...