Marine environments are a fruitful source of bioactive compounds some of which are the newest leading drugs in medicinal therapeutics. Of particular importance are organisms like sponges and macroalgae and their associated microbiome. Planctomycetes, abundant in macroalgae biofilms, are promising producers of bioactive compounds since they share characteristics, like large genomes and complex l...

OBJECTIVE To find anti-phytopathogen compounds from endophytic fungi associated with the endangered species Heptacodium miconioides. METHODS Fungi from H. miconioides with antifungal activities were isolated according to the plate growth inhibition method. The fungus with preferable antifungal activities was identified by morphological identification and 5. 8S rRNA sequence analysis. The bioa...

Wild-type cultures of Scytalidium flavo-brunneum produce a 15-azasterol antifungal agent and a reddish brown pigment as secondary metabolites. Spontaneous mutants of S. flavo-brunneum that had simultaneously lost the ability to produce the 15-azasterol and the pigment were transformed with plasmid pSFB-1, which was obtained from wild-type S. flavo-brunneum. Each transformant possessed the plasm...

The marine environment represents a largely untapped source for isolation of new microorganisms with potential to produce biologically active secondary metabolites. Among such microorganisms, Gram-positive actinomycete bacteria are of special interest, since they are known to produce chemically diverse compounds with a wide range of biological activities. We have set out to isolate and characte...

Fusarium head blight (FHB) is an important disease of wheat and causes significant economic and yield losses. The principal pathogen associated with FHB in Manitoba is Gibberella zeae (Schwein.) Petch (anamorph = Fusarium graminearum Schwabe). Trichoderma harzianum Rifai is a well-known biocontrol agent of many plant pathogens. The extracts from 11 T. harzianum isolates and cellfree filtrates w...

Two new antibiotic metabolites, dacrymenone (1) and VM 3298-2 (2), were isolated from fermentations of a Dacrymyces sp., and their structures were determined by spectroscopic techniques. Dacrymenone (1) is a new eremophilane sesquiterpenoid while VM 3298-2 (2) is a bis-enol ether between methyl pyruvate and 4,6-dihydroxycyclohexan-1,3-dione. Dacrymenone (1) shows weak antibacterial and antifung...

Insertional mutagenesis was applied for the first time to a fungal biocontrol agent, Pseudozyma flocculosa, in an attempt to obtain mutants with altered antagonistic properties. Transformants were obtained via DNA-mediated transformation. Molecular analyses of the transformants revealed that multiple copies of the plasmid were integrated in tandem at one to many chromosomal loci. The transforma...

The aim of this study was to create a new computational model capable of evaluating the affinity of imprinted materials to the specific target. A 1-(2,4-difluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethanone (L1), the main metabolite of voriconazole (L2)--a modern antifungal drug, was proposed as a template. In a computational analysis of polymerization systems composed of the template, the monomers...

AIMS The aim of this study was to evaluate the influence of environmental conditions on the antifungal activity of the Bacillus sp. CCMI 1053 cultures. METHODS AND RESULTS The electrospray ionization mass spectra (ESI-MS) analysis was used to detect the active peptides produced by Bacillus amyloliquefaciens CCMI 1051 cultures in a glucose-containing medium to which four different nitrogen sou...

and position of cyclization was then confirmed through peracylation and NMR spectroscopic analysis of peracylatedjagaricin. Knockout mutants were constructed to provide further confirmation that the jag genes were responsible for jagaricin production, and no jagaricin could be detected when the knockout mutant was grown on mushroom tissue and examined with IMS. Jagaricin’s native biological rol...