PURPOSE We evaluated the pharmacokinetics of intravitreally injected ranibizumab and aflibercept, and their effects on VEGF in the aqueous humor of vitrectomized and nonvitrectomized macaque eyes. METHODS Intravitreal ranibizumab (IVR; 0.5 mg/50 μL) or intravitreal aflibercept (IVA; 2 mg/50 μL) was injected into the previously vitrectomized right eyes of three macaques and nonvitrectomized ri...

PURPOSE To identify the molecules in normal human intraocular fluid (aqueous humor and vitreous) that inhibit the functional activity of the complement system. METHODS Aqueous humor and vitreous were obtained from patients with noninflammatory ocular disease at the time of surgery. Samples were incubated with normal human serum (NHS), and the mixture assayed for inhibition of the classical an...

Aqueous humor collected from freshly enucleated bovine eyes was passed over a gelatin affinity column, and bound material was eluted with 4M urea. When fractionated by sodium dodecyl sulfate electrophoresis, the eluted material co-migrated with human plasma fibronectin and protein standards of approximately 220,000 daltons. Amino acid analyses demonstrated a composition similar to that of human...

PURPOSE Inflammation generates changes in the protein constituents of the aqueous humor. Proteins that change in multiple models of uveitis may be good biomarkers of disease or targets for therapeutic intervention. The present study was conducted to identify differentially-expressed proteins in the inflamed aqueous humor. METHODS Two models of uveitis were induced in Lewis rats: experimental ...

To evaluate the diagnostic sensitivity of a panel of laboratory tests for ocular toxoplasmosis performed at the time of presentation, paired samples of aqueous humor and serum were collected from 49 consecutive episodes of ocular toxoplasmosis with a clinical course of less than 3 weeks. Total immunoglobulin G (IgG) and Toxoplasma gondii-specific IgG, IgM, and IgA were quantified by enzyme-link...

PURPOSE To determine the ability of the aqueous humor to generate and degrade H2O2, the effect of environmental factors such as oxygen tension and temperature, and the constituents contributing to the observed results. METHODS Aqueous humor was carefully removed from bovine eyes within 3 hours of death. Standard and new techniques were used to follow H2O2 degradation and formation. Catalase a...

The second annual ARVO/Pfizer Ophthalmic Research Institute conference was held Friday and Saturday, April 28 and 29, 2006, at the Fort Lauderdale Grande Hotel and Yacht Club, Fort Lauderdale, Florida. The conference, funded by The ARVO Foundation for Eye Research through a grant from Pfizer Ophthalmics, provided an opportunity to gather experts from within and outside ophthalmology to develop ...

The effect of plasma level of 5, 5-dimethyl-2, 4-oxazolidinedione (DMO) and of inhibitors of aqueous secretion on the distribution of DMO in intraocular fluids has been studied. Plasma levels of DMO from 35 to 60 mg./lOO ml. were required to obtain an appreciable excess of DMO in posterior and anterior aqueous humors compared with plasma. At plasma levels below 20 mg./lOO ml. the concentration ...

We compared both concentrations (0.125 and 1.0%) of two widely used commmerical brands of prednisolone acetate suspensions. We investigated the concentration that each product attained in the cornea and aqueous humor, and each product's anti-inflammatory effectiveness in the cornea. There was no statistically significant difference in the corticosteroid levels measured in cornea or aqueous humo...

4. Hallett, E.: A study of intraocular tension during general anesthesia, Tr. Ophth. Soc. XXIV: 73, 1965. 5. Sampaolesi, R., Reca, R., and Carro, A.: Personal communication, Oct., 1967. 6. de Roetth, A., Jr. and Schwartz, H.: Aqueous humor dynamics in glaucoma: Effect of ganglionic blocking agents and thiopental sodium anesthesia on aqueous humor dynamics, Arch. Ophth. 55: 755, 1956. 7. Apt, L....