the effect of ascorbic acid (aa) and butylated hydroxyanisole (bha) supplementation on aflatoxicosis in broiler chickens (1 to 42 days of age) was investigated (250, 500, 1000 and 2000 ppm for aa and bha) in diet containing 1 ppm total aflatoxin (af: 76.45% afb1, 10.52% afb2, 9.89% afg1 and 3.14% afg2). a total of 300 day-old broiler chicks were divided into ten treatment groups (t1=control; t2...

IN an earlier communication [Birch and Dann, 1933] on the estimation and distribution of ascorbic acid and glutathione in animal tissues we drew attention to the distribution of ascorbic acid in organs other than the suprarenal cortex, as detected by the indophenol reagent. The presence in a number of tissues of reducing substances other than glutathione had become evident from the work of Maso...

The first in vivo detection of a vitamin with nuclear magnetic resonance (NMR) is reported for mammalian liver. Vitamin C, also known as ascorbic acid, was monitored noninvasively in rat liver by "whole body"13C NMR spectroscopy at high field after infusion of [1,2-13C2]glucose into anesthetized rats. Generally, the carbon resonances of ascorbic acid overlap with those of other highly abundant ...

The importance of ascorbic acid for the metabolism of connective tissue substances, especially collagen, has been established by numerous investigators, using morphologic criteria (1). However, there have been few biochemical studies of this function of ascorbic acid with the exception of the recent analyses of Elster (2) and Robertson (3), which demonstrated that the maintenance of preformed c...

The transcriptional regulator UlaR belongs to the family of PRD-containing transcriptional regulators, which are mostly involved in the regulation of carbohydrate metabolism. The role of the transcriptional regulator UlaR in Streptococcus pneumoniae has recently been described [1]. Here, we report detailed genome-wide transcriptional profiling of UlaR-regulated genes in S. pneumoniae D39 and it...

The importance of ascorbic acid for the metabolism of connective tissue substances, especially collagen, has been established by numerous investigators, using morphologic criteria (1). However, there have been few biochemical studies of this function of ascorbic acid with the exception of the recent analyses of Elster (2) and Robertson (3), which demonstrated that the maintenance of preformed c...

Maximal aerobic capacity (Vo(2max)) decreases progressively with age, primarily because of a reduction in maximal cardiac output (Q(max)). This age-associated decline in Vo(2max) may be partially mediated by the development of oxidative stress that can suppress beta-adrenergic-receptor responsiveness and, consequently, reduce Q(max). To test this hypothesis, Vo(2max) (indirect calorimetry) and ...

Ascorbic acid is concentrated in various ocular compartments where it is thought to protect diurnal animal species against damaging effects of ultraviolet radiation. The authors evaluated the possibility that corneal endothelial cells have specific transport and/or metabolic properties that deliver ascorbic acid to the stroma. Bovine corneal endothelial cells were grown to confluence in multipl...

a sensitive procedure for determining total vitamin c (ascorbic acid + dehydrate ascorbic acid) in a blood drop from a finger prick, before and after the adminis- tration of a vitamin c tablet is described. analysis was carried out by high per- formance liquid  chromatography with  electrochemical detection (hplc/ec). measurements were taken one hour, two hours and six hours after the administr...

Neuroblastoma cells accumulate ascorbic acid and iron. It was hypothesized that these features could be exploited for sensitizing neuroblastoma cells for therapy in combination with reactive oxygen intermediates. In the present study the effects of 6-hydroxydopamine (6-OHDA) and H2O2 on metabolic parameters critical for cell survival were investigated in cells with low and high ferritin content...